BSL-2实验室开展SARS-CoV-2核酸检测过程中的生物安全管理

目的:总结生物安全二级(bio-safety level 2,BSL-2)实验室开展新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)核酸检测过程中的生物安全管理经验与效果。方法:检测样本来源于2020年2月21日到2020年11月5日送达中国疾控中心病毒病预防控制所病毒资源中心科室的人体咽拭子、鼻拭子、痰液、尿液、便液等。在遵循常规生物安全管理要求的基础上,在检测过程中积极进行人员、岗位、标本、过程、安全巡视等生物安全管理。结果:共开展了27692人次样本的SARS-CoV-2核酸检测,平均每天检测(2.32±0.11)批样本,每批平均检测(87.22±6.83)件样本,BSL-2实验室平均每天工作时间(3.43±0.23)h。在检测过程中未出现任何生物安全问题。结论:BSL-2实验室在长时间的SARS-CoV-2核酸检测过程中,要积极从多方面加强生物安全管理,从而保障检测工作安全有序进行。

Biosafety management of SARS-CoV-2 nucleic acid detection process in BSL-2 laboratory

Objective To summarize the experience and effectiveness of biosafety management for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid detection process in biosafety level 2(BSL-2)laboratories.Methods The samples were the human specimens received by the Department of Center for Virus Resource in the Viral Disease Control and Prevention Institute of China CDC from February 21,2020 to November 5,2020,including throat swabs,nasal swabs,sputum,urine and stool.In addition to routine biosafety management requirements,the biosafety management of personnel,positions,specimens,processes,and safety inspections during the testing process were actively conducted.Results A total of 27692 cases’samples were tested for SARS-CoV-2 nucleic acid.there were(2.32±0.11)batches of samples were tested per day,(87.22±6.83)samples were tested on average per batch and(3.43±0.23)h were spent in the BSL-2 laboratories per day.There were no biosafety issue occurred during the test process.Conclusions During the long-term SARS-CoV-2 nucleic acid detection process,the BSL-2 laboratory must actively strengthen biosafety management in many aspects to ensure the safe and orderly test of the work.