微小RNA-622对非小细胞肺癌A549细胞增殖、凋亡的影响

目的 探讨微小RNA (miRNA)-622对非小细胞肺癌A549细胞增殖、凋亡的影响。方法 培养A549细胞,分为miRNA-622组和miRNA-622阴性对照组 (NC组),分别转染miRNA-622模拟物及miRNA-622阴性对照。使用实时荧光定量聚合酶链反应(qPCR)检测miRNA-622组和NC组miRNA-622的表达水平,采用噻唑蓝实验检测两组细胞增殖能力,采用流式细胞学技术检测两组细胞凋亡率。结果 miRNA-622组和NC组细胞中miRNA-622的相对表达水平分别为0.993±0.058、0.631±0.053,OD值分别为0.631±0.049、0.922±0.058,细胞凋亡率分别为12.230%±0.176%、7.400%±0.208 %,miRNA-622组miRNA-622的相对表达水平和细胞凋亡率均高于NC组,而OD值低于NC组,差异均有统计学意义(t=23.650、17.710、3.822,P值均<0.01)。结论 miRNA-622可抑制A549细胞增殖,促进A549细胞凋亡。

Effects of microRNA-622 on the proliferation and apoptosis of non-small cell lung cancer A549 cells

Objective To investigate the effect of microRNA (miRNA)-622 on the proliferation and apoptosis of non-small cell lung cancer A549 cells.Methods A549 cells were cultured and divided into miRNA-622 group and miRNA-622 negative control group (NC group) transfected with miRNA-622 mimics and miRNA-622 negative controls, respectively. MiRNA-622 expression was detected using quantitative real-time polymerase chain reaction, cell proliferation was analyzed through methyl thiazolyl tetrazolium assay, and cell apoptosis rate was examined through flow cytometry.Results The miRNA-622 and NC groups had relative miRNA-622 expression levels of 0.993±0.058 and 0.631±0.053, respectively; OD values of 0.631±0.049 and 0.922±0.058, respectively; and apoptosis rates of 12.230%±0.176% and 7.400%±0.208%, respectively. The miRNA-622 group had higher relative miRNA-622 expression and apoptotic rate but lower OD value than the NC group, and the differences were statistically significant (t=23.650, 17.710, 3.822, all P values<0.01).Conclusions miRNA-622 can inhibit the proliferation and promote the apoptosis of A549 cells.