可变数串联重复序列检测异基因外周血造血干细胞移植后植？…

目的 从血型、染色体核型、ＤＮＡ可变数串联重复序列（ＶＮＴＲ）几个方面进行对比研究以更好地评估异基因移植后的植入状态及其与疾病复发的关系。方法 采用聚合酶链反应（ＰＣＲ）方法扩增Ｄ１７Ｓ３０、Ｄ１Ｓ８０、ＡｐｏＢ３个不同位点的多态性,对１５例白血病患者异基因外周血造血干细胞移植（ａｌｌｏ－ＰＢＳＣＴ）后的嵌合状态进行检测,对其中７例异性间移植和３例血型不合移植又分别进行了染色体核型分析和全套血型分

Variable number of tandem repeats as an evidence of engraftment status after allogeneic peripheral blood stem cell transplantation

Objective To evaluate engraftment status of patients after allogeneic peripheral blood stem cell transplantation (allo PBSCT) based on red blood cell type, karyotype and variable number of tandem repeats (VNTR). Methods Amplification of 3 loci of VNTR in vitro by polymerase chain reaction (PCR) was preformed to detect the polymorphism of 15 patients post PBSCT. Furthermore, analysis of karyotype in 7 sex mismatched patients and red blood cell antigen switching in 3 ABO blood type mismatched patients were also employed. Results VNTR analysis in 15 patients revealed complete chimerism in 11 patients and mixed chimerism in 4. In the 7 sex mismatched patients, karyotype analysis showed types of their donors. As for blood type analysis, it was after 2 months that the first patient transformed to his donor's type. One patient showed mixed status 7 months after transplantation. Conclusion Amplification of VNTR loci by PCR for documenting engraftment status after allo PBSCT is simple and sensitive. This technology is especially suitable for patients who have received stem cells with the same sex, same blood type and identical HLA type. Examination with several different VNTR loci will improve the power of discrimination.