Fischer344大鼠上皮性卵巢癌动物模型的建立

目的建立类似人卵巢癌临床表现的大鼠上皮性卵巢癌动物模型。方法将大鼠上皮性卵巢癌NuTu19细胞按不同细胞数接种于同种Fischer344大鼠腹腔内,观察其体质量、生存期的改变;接种细胞4周、8周、16周后,系统解剖接种后大鼠并做常规病理学检查。结果接种后大鼠半数生存期:细胞数为5×106组为30d;1×106组62d;5×105组66d;1×105组129d;5×104组160d;1×104组223d;所有模型大鼠死亡前3周体质量均上升,并出现腹水;出现与人卵巢癌晚期类似临床表现,如血性腹水,腹膜和横膈、盆腔腹腔器官表面接种粟粒状结节,大小网膜呈饼状。组织学检查结果为浆液性乳头状囊腺癌,但卵巢组织在晚期才表现为癌症浸润。结论Fischer344大鼠上皮性卵巢癌动物模型在肿瘤生长方式、疾病进展过程、组织学类型、并发症等方面与人卵巢癌类似,是较为理想的卵巢癌动物模型。

Establishment of Fisher344 Rat Animal Model of Epithelial Ovarian Cancer

Objective To establish rat model of epithelial ovarian cancer which clinical character should be similar to human ovarian cancer. Methods Epithelial ovarian cancer cells(NuTu19) were injected intraperitoneally into Fischer344 rat in different cell number. Weight and survival time were observed weekly. Necropsy and pathologic analysis were performed after 4, 8, 16 weeks. Results Mean survival time: 5×10⁶ group was 30 d, 1×10⁶ group was 62 d, 5×10⁵ group was 66 d, 1×10⁵ group was 129 d, 5×10⁴ group was 160 d, 1×10⁴ group was 223 d. Body weight of all rats were raised and ascites were found at three weeks before death. Clinical characters were similar to human ovarian cancer: hemorrhagic ascites, confluent omental mass, peritomeum, diaphragm and intraabdominal organs were implanted numerous tumor nodules. Histological character was papillary serous ovarian adenocarcinoma. Only in terminal stages, ovarian tissues were affected. Conclusion The model is consistent with clinical human ovarian cancer in growth manner, development of disease process, histological type and death complications.