Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms |
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| Authors: | van de Pol Alma C van Loon Anton M Wolfs Tom F W Jansen Nicolaas J G Nijhuis Monique Breteler Els Klein Schuurman Rob Rossen John W A |
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| Affiliation: | Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. |
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| Abstract: | Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively. |
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