| 反义核酸对膀胱癌细胞系多药耐药性的逆转效应 |
| |
| 引用本文： | 何斌,杨唐俊,金锡御. 反义核酸对膀胱癌细胞系多药耐药性的逆转效应[J]. 中华泌尿外科杂志, 2000, 21(2): 69-71 |
| |
| 作者姓名： | 何斌 杨唐俊 金锡御 |
| |
| 作者单位： | 第三军医大学附属新桥医院泌尿外科(何斌!400037重庆，杨唐俊!400037重庆，金欢胜!400037重庆，王祥卫!400037重庆，刘素英!400037重庆，曾毅!400037重庆)，第三军医大学(金锡御!400037重庆) |
| |
| 基金项目： | 国家自然科学基金!资助（39670824） |
| |
| 摘 要： | 目的 探讨多药耐药性逆转的新方法。方法 应用基因重组方法构建能转录出ｍｄｒ１ ｍＲＮＡ反义ＲＮＡ的逆转录病毒质粒，然后将该质粒导入膀胱癌耐药细胞系中，测定转染细胞对不同药物的耐药性，观察反义核酸对ＭＤＲ的逆转效应。结果 耐药细胞经导入反义核酸逆转录病毒质粒后，对阿霉素（ＡＤＭ）、秋水仙素（ＣＯＬ）的ＩＣ５０显著降低（Ｐ〈０．０１）；细胞膜上Ｐ－糖蛋白（Ｐ－ＧＰ）染色信号显著减弱。结论 反义核酸是逆
|
| 关 键 词： | 膀胱肿瘤 多药耐药性 反义核酸 逆转效应 |
Reversal of multidrug resistance of bladder cancer cell line with antisenes RNA |
| |
| HE Bin,YANG Tangjun,JIN Xiyu,et al.. Reversal of multidrug resistance of bladder cancer cell line with antisenes RNA[J]. Chinese Journal of Urology, 2000, 21(2): 69-71 |
| |
| Authors: | HE Bin YANG Tangjun JIN Xiyu et al. |
| |
| Affiliation: | HE Bin,YANG Tangjun,JIN Xiyu,et al. Department of Urology,the Affiliated Xinqiao Hospital of the Third Military Medical University,Chongqing 400037,China |
| |
| Abstract: | Objective To explore the new reversal method of multidrug resistance of bladder cancer cell line. Methods A retroviral plasmid pLXSN AS( ) containing the mdr1 mRNA antisense gene was cloned by inserting 1383bp fragment of mdr1 cDNA into multiple cloning sites of retroviral vector pLXSN. For evaluating the reversal efficiency of antisense RNA the recombinant plasmid pLXSN AS( ) was transfected into multidrug resistant cell lines BHA 60 with liposome. Results After the recombinant plasmid pLXSN AS( ) being transduced, the BHA 60 cell line showed significant decrease of IC 50 to drug ( P < 0.01) and the P GP positive signal. Conclusions Antisense is a valuable method to reverse multidrug resistance of bladder cancer cell line. |
| |
| Keywords: | Bladder neoplasms Carcinoma Multidrug resistance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
