哮喘大鼠脑内小胶质细胞激活的时程及形态变化研究

为探讨哮喘大鼠在连续激发不同阶段脑内小胶质细胞激活的时程及形态变化,本实验取健康雄性SD大鼠制备哮喘模型并诱发哮喘发作。48只SD大鼠随机分为对照组和实验组。对照组分为正常组和生理盐水组(n=8);实验组根据造模期间连续抗原激发的时间不同,分为连续激发3,7,14,21d组(n=8)。各组大鼠均进行呼吸功能检测、肺组织切片HE染色及脑组织抗OX-42免疫组织化学染色。结果显示:与对照组大鼠比较,实验组中各组大鼠呼吸功能各项指标均明显加重;肺组织病变程度逐渐增加;小胶质细胞由静息状态转变为激活状态,且数量明显增多。以上结果提示:哮喘大鼠脑内小胶质细胞被激活,在一定时间内小胶质细胞激活的程度随呼吸功能的降低及肺组织病变程度的增加而增加。

The study of morphological change and time course of microglial activation in the rat brain of asthma model

To investigate changes in morphology and time course of activated micrnglia in asthma rats, the healthy male SD rats were used and prepared asthma models induced by continuous antigen stimulation. Forty-eight SD rats were randomly divided into eontrol group and experimental group. Control group was divided into normal group and saline group (n =8) ; experimental group was divided into 3 d,7 d, 14 d and 21 d groups according to the different duration time of antigen stimulation (n = 8). Every rat was detected for the respiratory function, accessed lungs tissue for HE staining and brain tissue for OX-42 immunohiatochemical staining. The results showed that,com-pared with the control group, rats of experimental group had more severe respiratory function, and lesions in lungs tissue became more and more serious, and microglia gradually changed from resting state to activated state and the number was markedly increased. The above re-suits suggest that microglias in asthma rat brain are activated; activation of microglia with the extent of severe respiratory function and le-sions in lungs tissue is increased in a certain period of time.