| 高效液相色谱－蒸发光散射检测器法测定复方桔梗止咳片中桔梗皂苷D含量 |
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| 引用本文： | 陈国宁,;邰顺章,;黄柳燕.高效液相色谱－蒸发光散射检测器法测定复方桔梗止咳片中桔梗皂苷D含量[J].中国药业,2014(18):51-53. |
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| 作者姓名： | 陈国宁 ;邰顺章 ;黄柳燕 |
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| 作者单位： | [1]江苏省盐城市第二人民医院,江苏盐城224003; [2]江苏省盐城市药品检验所,江苏盐城224002; [3]盐城师范学院,江苏盐城224002 |
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| 摘 要： | 目的建立测定复方桔梗止咳片中桔梗皂苷D含量的高效液相色谱－蒸发光散射检测器（HPLC—ELSD）法。方法色谱柱采用Agilent C18柱（150mm×4．6mm，5μm），以乙腈-水（25：75）为流动相，流速为0．7mL／min；ELSD检测器漂移管温度为90℃，载气1．8Bar；进样量为10μL；柱温为30℃。结果桔梗皂苷D质量浓度线性范围为125．08—1250．8mg／L（r=0．9998），平均加样回收率为98．60％，RSD=1．00％（n=6）。结论该方法简便、重复性好，可用于测定复方桔梗止咳片中桔梗皂苷D的含量。
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| 关 键 词： | 复方桔梗止咳片 桔梗皂苷D 高效液相色谱-蒸发光散射检测器 含量测定 |
Content Determination of Platycodin D Content in Compound Radix Platycodi Anticough Tablets by HPLC- ELSD |
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| Institution: | Chen Guoning, Tai Shunzhang, Huang Liuyan (1. Yancheng Municipal Second People＇s Hospital, Yancheng, Jiangsu, China 224003; 2. Yancheng Municipal Institute for Drug Control, Yancheng, Jiangsu, China 224002; 3. Yancheng Normal College, Yancheng, Jiangsu, China 224002) |
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| Abstract: | Objective To establish a quantitative method for the determination of platycodin D content in Compound Radix Platycodi Anticough Tablets by high performance liquid chromatography coupled with ELSD（HPLC-ELSD）.Methods The Agilent C18 column（150mm×4.6mm,5μm） was adopted. The mobile phase consisted of acetonitrile-water（25：75） at a flow rate of 0. 7 mL/min,ELSD detector was used,the drift tube temperature was 90 % ,the carrier gas 1. 8 Bar;the sample quantity： 10 μL;the column temperature 30 ℃. Results The mass concentration curve showed a good linearity in the ranges of 125.08-1 250.8 mg/L（r=0.999 8）.The average recovery rate was 98.60%, RSD = 1.00% （n=6）. Conclusion The method is simple and reproducible,which can be used for the quantitative determination of platycodin D in Compound Radix Platycodi Anticough Tablets. |
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| Keywords: | Compound Radix Platycodi Anticough Tablets platycodin D HPLC - ELSD content determination |
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