| Peroxynitrite induced decrease in Na^＋, K^＋-ATPase activity is restored by taurine |
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| 引用本文： | Kocak-Toker N,Giris M,Tülübas F,Uysal M,Aykac-Toker G. Peroxynitrite induced decrease in Na^＋, K^＋-ATPase activity is restored by taurine[J]. World journal of gastroenterology : WJG, 2005, 11(23): 3554-3557. DOI: 10.3748/wjg.v11.i23.3554 |
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| 作者姓名： | Kocak-Toker N Giris M Tülübas F Uysal M Aykac-Toker G |
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| 作者单位： | Department of Biochemistry,Department of Biochemistry,Department of Biochemistry,Department of Biochemistry Istanbul Faculty of Medicine,Istanbul University,Capa 34093,Istanbul,Turkey,Istanbul Faculty of Medicine,Istanbul University,Capa 34093,Istanbul,Turkey,Istanbul Faculty of Medicine,Istanbul University,Capa 34093,Istanbul,Turkey,Istanbul Faculty of Medicine,Istanbul University,Capa 34093,Istanbul,Turkey |
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| 基金项目： | Supported by the Istanbul University Research Foundation,No.BYP-247/20082003 |
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| 摘 要： | AIM: Peroxynitrite (ONOO-) is a powerful oxidant shown to damage membranes. In the present study, the effect of taurine on changes of liver plasma membrane Na+, K+-ATPase induced by ONOO- was investigated. METHODS: Liver plasma membrane was exposed to ONOO-with or without taurine. Na+, K+-ATPase activity and lipid peroxidation as thiobarbituric acid reactive substances (TBARS) levels were measured. RESULTS: Different concentrations of ONOO- (100, 200, 500, and 1 000 μmol/L) were found to decrease liver plasma membrane Na+, K+-ATPase activity significantly. The depletion of enzyme activity was not concentration dependent. Effects of different concentrations of taurine on liver plasma membrane Na+, K+-ATPase activity were also measured. Taurine did not cause any increase in enzyme activity. When plasma membranes were treated with 200 μmol/L ONOO- with different concentrations of taurine, a restoring effect of taurine on enzyme activity was observed. TBARS levels were also measured and taurine was found to decrease the elevated values. CONCLUSION: Taurine is observed to act as an antioxidant of ONOO- to decrease lipid peroxidation and thus affect liver plasma membrane Na+, K+-ATPase by restoring its activity.
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| 关 键 词： | 氧化剂 钠离子 钾离子 ATP酶 酶活性 肝脏损伤 |
| 收稿时间： | 2004-07-23 |
Peroxynitrite induced decrease in Na+, K+-ATPase activity is restored by taurine |
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| Kocak-Toker Necla,Giris Murat,Tülübas Feti,Uysal Müjdat,Aykac-Toker Gülcin. Peroxynitrite induced decrease in Na+, K+-ATPase activity is restored by taurine[J]. World journal of gastroenterology : WJG, 2005, 11(23): 3554-3557. DOI: 10.3748/wjg.v11.i23.3554 |
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| Authors: | Kocak-Toker Necla Giris Murat Tülübas Feti Uysal Müjdat Aykac-Toker Gülcin |
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| Affiliation: | partment of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa 34093, Istanbul, Turkey |
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| Abstract: | AIM: Peroxynitrite (ONOO-) is a powerful oxidant shown to damage membranes. In the present study, the effect of taurine on changes of liver plasma membrane Na+, K+-ATPase induced by ONOO- was investigated. METHODS: Liver plasma membrane was exposed toONOO-with or without taurine. Na+, K+-ATPase activity and lipid peroxidation as thiobarbituric acid reactive substances (TBARS) levels were measured.RESULTS: Different concentrations of ONOO- (100, 200,500, and 1 000 μmol/L) were found to decrease liver plasma membrane Na+, K+-ATPase activity significantly. The depletion of enzyme activity was not concentration dependent. Effects of different concentrations of taurine on liver plasma membrane Na+, K+-ATPase activity were also measured. Taurine did not cause any increase in enzyme activity. When plasma membranes were treated with 200 μmol/L ONOO- with different concentrations of taurine, a restoring effect of taurine on enzyme activity was observed. TBARS levels were also measured and taurine was found to decrease the elevated values. CONCLUSION: Taurine is observed to act as an antioxidant of ONOO-to decrease lipid peroxidation and thus affect liver plasma membrane Na+, K+-ATPase by restoring its activity. |
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| Keywords: | Na+ K+-ATPase Taurine Peroxynitrite |
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