艾滋病相关淋巴结病理改变及其与淋巴结中CD4+CD25+调节性T细胞的相关性

目的探讨艾滋病患者淋巴结组织中CD4 CD25 调节性T细胞的表达、分布及其与病理改变的相关性。方法对22例活检及13例尸体解剖的HIV/AIDS患者的淋巴结进行组织学观察和病理分期,采用免疫组织化学法对淋巴结组织中的CD4 CD25 调节性T细胞特异性标记物FoxP3进行检测。结果35例艾滋病患者的淋巴结中,1～4期分别有5、4、14和12例。所有淋巴结组织中均检测出FoxP3的阳性表达;在1、2期病变淋巴结内,FoxP3阳性细胞数量较多,分布于滤泡间区和副皮质区;3、4期随着淋巴细胞的数量衰竭,阳性细胞数量的减少更加明显。结论艾滋病患者淋巴结中存在有CD4 CD25 调节性T细胞,随着淋巴结病变的进展其数量减少或耗竭。

Pathology of AIDS-related lymphadenopathy and its relationship to expression of CD4 + CD25 + regulatory T cells in lymphoid node tissue

Objective To observe the pathology of AIDS-related lymphadenopathy and its relationship to the expression and distribution of CD4 CD25 regulatory T cells in lymphoid node tissue.Methods Totally 22 biopsy and 13 autopsy lymphoid node tissues from HIV-positive patients were examined under microscopy and pathological staging was performed.Specific marker for CD4 CD25 regulatory T cells in lymphoid node tissue was detected with anti-Foxp3 monoclonal antibody by immunohistochemistry.Results Among all the 35 specimens,5,4,14,and 12 specimens were histopathologically staged from 1 to 4,respectively.FoxP3 were detected in all lymphoid node tissues.The distribution of FoxP3-positive lymphocytes were mainly in intermediate zone of follicle and cortical area in stages 1 and 2.The counts of FoxP3-positive lymphocytes remarkedly decreased in stages 3 and 4,following depletion of lymphocytes.Conclusions CD4 CD25 regulatory T cells exist in lymphoid node tissue of patients with HIV infection.Their amounts decrease or deplete along with the progression of AIDS-related lymphadenopathy.