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Development of a Solid-Phase Radioimmunoassay for the Determination of Arginine-Vasopressin in Urine |
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| Authors: | P. Larose J. Gutkowska P. du Souich H. Ong |
| Affiliation: | 1. Faculty of Pharmacy , University of Montreal , P.O. Box 6128, Montreal, Canada , H3C 3J7;2. Clinical Research Institute of Montreal , 110 W PineAve, Montreal;3. Department of Pharmacology , University of Montreal , P.O. Box 6128, Montreal, Canada , H3C 3J7 |
| Abstract: | A new solid-phase radioimmunoassay (RIA) has been developed for measuring arginine-vasopressin (AVP) in urine. AVP is first extracted from urine by adsorption on Vycor glass powder and eluted with acetone-water (60:40). The mean recovery is 75.3 ± 2.2% (n =18). The organic extract is evaporated to dryness and reconstituted in the assay buffer. Aliquots of this extract are then incubated with 125I-AVP in polystyrene LKB tubes previously coated with the antiserum (1:50000) for 48 hours. The free radioactive fraction is removed by aspiration and the tubes are counted. Values correlate well with those obtained by liquid-phase RIA using dextran-charcoal. Urinary AVP concentrations in normal Sprague-Dawley rats and rats with varying degrees of hydration have been measured. |
| Keywords: | arginine-vasopressin solid-phase radioimmunoassays hormone assays neuropeptides peptide hormones urine extracts |