| Sysmex XE-2100对未成熟粒细胞临界值的确定 |
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| 引用本文： | 吴军录,戴燕,万海英. Sysmex XE-2100对未成熟粒细胞临界值的确定[J]. 检验医学与临床, 2011, 8(23): 2822-2823,2825. DOI: 10.3969/j.issn.1672-9455.2011.23.003 |
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| 作者姓名： | 吴军录 戴燕 万海英 |
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| 作者单位： | 同济大学附属同济医院检验科,上海,200065 |
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| 摘 要： | 目的对Sysmex XE-2100全自动血细胞分析仪的白细胞分类报警系统(Q-Flags)中提示的"未成熟粒细胞?(Imm Gran?)"报警信息的可信度进行分析,选择最佳临界值以确定手工镜检复查的标准。方法选择本院门、急诊及住院患者的1 200例标本,根据仪器分类报警系统中"Imm Gran?"不同提示值的标本进行分类、染色、镜检,计数200个白细胞,以查见原始和幼稚细胞细胞大于或等于1%,早幼粒和中幼粒细胞大于或等于1%,晚幼粒细胞大于2%为阳性标准。把Imm Gran的临界值分别设为10、20、30……290、300,通过数据分析确定最佳临界值。其中假阴性率的最大可接受限不能超出5%。最后选择744例标本验证新确定临界值的可信度。结果 (1)通过比对确定临界值为150是最佳临界值。修改后,在假阴性不超过5.00%(4.51%)的情况下,假阳性率由56.36%降到35.64%,真阴性率由43.64%提高到64.36%,总符合率由57.00%提高到71.83%。(2)验证试验显示无初发白血病细胞漏检。结论 (1)重新建立了合适的Imm Gran临界值后,在保证检验质量的同时,减少了镜检复片数量,又提高了工作效率。(2)Imm Gran报警提示值在150～300范围时应进行手工分片镜检复查。(3)Q-Flags中临界值应根据自己实验室的实际情况重新修定。
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| 关 键 词： | 血常规 Sysmex XE-2100全自动血细胞分析仪 报警信息 临界值 |
The ascertainment of the threshold value of naive neutrophil in Sysmex XE-2100 |
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| WU Jun-lu,DAI Yan,WAN Hai-ying. The ascertainment of the threshold value of naive neutrophil in Sysmex XE-2100[J]. Laboratory Medicine and Clinic, 2011, 8(23): 2822-2823,2825. DOI: 10.3969/j.issn.1672-9455.2011.23.003 |
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| Authors: | WU Jun-lu DAI Yan WAN Hai-ying |
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| Affiliation: | WU Jun-lu,DAI Yan,WAN Hai-ying(Department of Clinical Laboratory,Tongji Hospital,Tongji University,Shanghai 200065,China) |
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| Abstract: | Objective To select the best threshold value to define the standard of manual re-examination by reliability analysis of naive neutrophil?(Imm Gran?) alarm information presented in leukocyte differential alarm system(Q-Flags) on the Sysmex XE-2100.Methods 1 200 cases were included in this study.Samples were processed with differential counting,staining,and microscope examination according to Imm Gran? information of Q-Flags.200 leukocytes were counted in a randomly selected portion of the slide under light ... |
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| Keywords: | blood routine test Sysmex XE-2100 alarm information threshold value |
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