| 黄芩素对4种肝损伤物质造成肝细胞毒性的拮抗作用 |
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| 引用本文： | 蒋萍,杨阳,季莉莉,王峥涛.黄芩素对4种肝损伤物质造成肝细胞毒性的拮抗作用[J].中国实验方剂学杂志,2012,18(6):145-148. |
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| 作者姓名： | 蒋萍 杨阳 季莉莉 王峥涛 |
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| 作者单位： | 1. 上海中医药大学中药研究所中药标准化教育部重点实验室,中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201210
2. 上海中医药大学中药研究所中药标准化教育部重点实验室,中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201210;上海中药标准化研究中心,上海201210 |
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| 基金项目： | 上海市科技启明星计划(跟踪) (10QH1402200);国家自然科学基金项目 (青年基金30801544) |
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| 摘 要： | 目的:观察黄芩素对4种肝毒性物质诱导的人正常肝细胞株L-02细胞损伤的拮抗作用.方法:培养的L-02细胞分别给予10 mmol· L-1对乙酰氨基酚(AP),50 μmol·L -1山岗橐吾碱( clivorine,CLI),2μmol·L-1川楝素( toosendanin,TSN)和100 mmol· L-1乙醇( EtOH)诱导肝细胞毒性.黄芩素1,10,25,50,100 μmol· L-1分别与细胞预孵15 min后,加入肝毒性物质,48 h后MTT法检测细胞存活率.结果:与对照组相比,4种肝毒性物质均能显著降低肝细胞存活率(P ＜0.001).经不同浓度黄芩素处理后,各浓度黄芩素均能显著提高AP,TSN,EtOH肝细胞损伤模型组的细胞存活率(P ＜0.01,P＜0.001)；10,25,50,100μmol·L-1黄芩素能显著提高CLI肝细胞损伤模型组的细胞存活率(P ＜0.05,P＜0.001).结论:黄芩素具有拮抗对乙酰氨基酚、山岗橐吾碱、川楝素和乙醇这些肝毒性物质诱导的肝细胞毒性的作用,且具有一定的浓度依赖性.
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| 关 键 词： | 黄芩素 肝毒性 肝细胞 |
| 收稿时间： | 9/7/2011 12:00:00 AM |
Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity |
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| JIANG Ping,YANG Yang,JI Li-li and WANG Zheng-tao.Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity[J].China Journal of Experimental Traditional Medical Formulae,2012,18(6):145-148. |
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| Authors: | JIANG Ping YANG Yang JI Li-li and WANG Zheng-tao |
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| Institution: | The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 201210, China;The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 201210, China;The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 201210, China; Shanghai R&D Center for Standardization of TCM, Shanghai 201210, China;The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 201210, China; Shanghai R&D Center for Standardization of TCM, Shanghai 201210, China |
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| Abstract: | Objective:The present study is designed to observe the protective effects of baicalein against hepatotoxins-induced cytotoxicity on the human normal liver L-02 cells.Method:L-02 cells were seeded and pretreated with or without baicalein(1,10,25,50,100 μmol·L-1) for 15 min,and then cells were further treated with or without various hepatotoxins,including acetaminophen(AP,10 mmol·L-1),clivorine(CLI,50 μmol·L-1),toosendanin(TSN,2 μmol·L-1) and ethanol(EtOH,100 μmol·L-1) for 48 h.The survival cells were determined by MTT assay.Result:Four hepatotoxins could significantly reduce the cell viability(P< 0.001) compared with the control group.After treatments,baicalein could increase the cell viability which was decreased by AP,TSN and EtOH(P<0.01,P<0.001).Moreover,baicalein could significantly increase the cell viability which was decreased by CLI(P<0.05,P<0.001).Conclusion:Baicalein can prevent the cytotoxicity induced by those hepatotoxins in human normal liver L-02 cells. |
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| Keywords: | baicalein hepatotoxicity hepatocyte |
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