草血竭纯化部分对小鼠甲型H1N1流感的疗效

目的探讨草血竭纯化部分对于小鼠甲型H1N1流感的疗效。方法采用滴鼻法建立甲型H1N1流感病毒感染小鼠模型,通过灌胃途径给予不同剂量的草血竭,以给予奥司他韦者为阳性对照,以未给予药物者为病毒对照组。观察14d内小鼠生活状态,计算小鼠肺指数,检测肺组织内流感病毒核酸相对量和病毒滴度。结果甲型H1N1流感病毒对小鼠的半数致死量为10-6。阳性对照组和草血竭高、中剂量组小鼠的存活率均为100%,平均生存日数均为14d,草血竭低剂量组小鼠的存活率为42.86%,平均生存日数为9.14d,而病毒对照组小鼠的存活率及平均生存日数分别为0和3.57d。不同组别小鼠平均生存日数比较,差异有统计学意义(F=8.53,P=0.02);阳性对照组、高剂量组、中剂量组、低剂量组小鼠平均生存日数均高于病毒对照组(P0.05)。阳性对照组和草血竭高剂量、中剂量、低剂量组及病毒对照组肺指数分别为:(0.84±0.07)%、(0.81±0.07)%、(0.84±0.10)%、(0.84±0.10)%和(1.42±0.12)%,差异有统计学意义(P=0.03);病毒核酸相对量分别为:0.37±0.11、0.42±0.06、0.42±0.06、0.65±0.19和1.00±0.06,差异有统计学意义(P=0.04);病毒滴度分别为:2.49±0.12、2.35±0.29、2.43±0.15、2.46±0.12和2.80±0.16,差异有统计学意义(P=0.04)。阳性对照组和草血竭各剂量组的肺指数、肺组织内流感病毒核酸相对量和病毒滴度均低于病毒对照组(P0.05);而阳性对照组的上述指标与草血竭各剂量组比较,差异无统计学意义(P0.05)。结论草血竭纯化部分在体内对甲型H1N1流感病毒具有抑制作用,能够改善甲型H1N1流感小鼠的肺组织的病变,降低病死率。

Therapeutic effect of extract of Polygonum paleaceum Wall on influenza A H1N1 virus infection in Balb/c mice

ObjectiveTo assess therapeutic effect of extract of Polygonum paleaceum Wall（PPW） on influenza A H1N1 virus infection in Balb/c mice. MethodsMice model of influenza A H1N1 virus infection was established by intranasal method, different doses of PPW were given by gavage, positive control group was given oseltamivir, and mice without giving oseltamivir were in virus control group. Life status of mice within 14 days was observed, lung index of mice was calculated, relative amount of viral nucleic acid and virus titer in lung tissue were detected. ResultsThe median lethal dose of influenza A H1N1 virus to mice was 10-6. Survival rates of mice in positive control group, high PPW dose group, and middle PPW dose group were all 100%, the average survival time was 14 days, survival rate and average survival time of mice in low PPW dose group were 42.86% and 9.14 days respectively, while in virus control group were 0 and 3.57 days respectively. The average survival days of mice in different groups were statistically significant (F=8.53, P=0.02); average survival days of mice in positive control group, high PPW dose group, middle PPW dose group, and low PPW dose group were all higher than those of virus control group (P<0.05). Lung index of positive control group, high PPW dose group, middle PPW dose group, low PPW dose group, and virus control group were (0.84±0.07)%, (0.81±0.07)%, (0.84±0.10)%, (0.84±0.10)%, and (1.42±0.12)% respectively, difference was statistically significant（P=0.03）; relative amount of viral nucleic acid were 0.37±0.11, 0.42±0.06, 0.42±0.06, 0.65±0.19, and 1.00±0.06 respectively, difference was statistically significant（P=0.04）; titer of virus were 2.49±0.12, 2.35±0.29, 2.43±0.15, 2.46±0.12, and 2.80±0.16 respectively, difference was statistically significant（P=0.04）. Lung index, relative amount of influenza virus nucleic acid and virus titer in lung tissue of positive control group and different PPW dose groups were all lower than that of virus control group (P<0.05); but the above indexes in positive control group were not statistically significant compared with different PPW dose groups (P>0.05).ConclusionExtract of PPW has inhibitory effect on influenza A H1N1 virus in vivo, it can decrease lung tissue lesions and reduce the mortality of mice infected with influenza A H1N1 virus.