淋巴细胞在慢性鼻-鼻窦炎组织中的表达及意义

目的：探讨B淋巴细胞（CD20）和T淋巴细胞亚群（CD4、CD8）在慢性鼻-鼻窦炎（CRs）中的表达及在炎症反应中的作用。方法：采用免疫组织化学技术检测B淋巴细胞（CD20）和辅助性T细胞（CD4）、细胞毒性T细胞（CD8）在实验组[分为15例CRS不伴鼻息肉（CRSsNP）组、12例CRS伴鼻息肉（CRSwNP）组、7例复发性CRSwNP组]和对照组（13例下鼻甲黏膜对照）中的表达。采用Mann-WhitneyU检验分析实验组和对照组B淋巴细胞、T淋巴细胞亚群的表达情况，实验组组间淋巴细胞浸润比较用单因素方差分析One—WayANOVA。结果：与对照组相比，实验组有明届的13淋巴细胞（CD20）和T淋巴细胞亚群（CD4、CD8）浸润（P〈0．05）：T淋巴细胞、亚群（CD4、CD8）在CRSwNP组和复发性CRSwNP组病变中的表达明显高于CRSsNP组（P〈0．05）；CD4在复发性CRSwNP组中的表达明显高于CRSwNP和CRSsNP组（P〈0．01）。结论：实验组鼻腔黏膜中B淋巴细胞、辅助性T细胞和细胞毒性T细胞高表达，均参与炎症的形成，其炎症匣应与T淋巴细胞浸润密切相关。

Expression and significance of lymphocytes in chronic rhinosinusitis

Ohjective：To investigate the B lymphocytes（CD20） and T lymphocyte subsets（CD4, CD8） expres sion and clinical significance in chronic rhinosinusitis. Method： Immunohistochemical technique was applied to de tect the expression of the B lymphocytes （CD20）, helper T lymphocytes （ CD4 ） and the cytotoxic T lymphocyte （CD8）in 15 patients with chronic rhinosinusitis without nasal polyps group（CRSsNP）, 12 patients of chronic nasal sinusitis with nasal poIyp（CRSwNP）, 7 cases of recurrent CRSwNP group and 1,3 patients with inferior turhinate mucosa in the control group. The Mann - Whitney U test was used to analyze CD20, CD4 and CD8 hetween the experimental groups and the control group. One-Way ANOVA was used to compare the lymphocyte infiltration between the experiment groups. Result： Patients with CRS had higher infiltration of the B lymphocytes（CD20）and T lymphocyte subsets（CD4,CDS）than the patienls of the control group（P〈0.05）. The expression of T lymphocyte subsets（CD4, CC8） in CRSwNP and recurrent CRSwNP groups was significantly higher than in CRSsNP （P〈0.05）. The expression of （＇D4 in the recurrent CRSwNP group was obviously higher than that in CRSsNP and CRSwNP（P〈0.01 ）. Conclusion： The B lymphocytes, helper T cells and cytotoxic T cells have the high ex pression in CRS groups, which involved with the formation of inflammation. Furthermore nasal polyps are closely related to T lymphocyte infiltration.