| 3,5-二硝基水杨酸（DNS）法测定五味子还原糖含量的适宜条件 |
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| 引用本文： | 江建丽.3,5-二硝基水杨酸（DNS）法测定五味子还原糖含量的适宜条件[J].海峡药学,2014,26(1):57-60. |
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| 作者姓名： | 江建丽 |
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| 作者单位： | 江建丽 (福建省南平市药品检验所,南平,353000)； |
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| 摘 要： | 目的 探讨3,5-二硝基水杨酸（DNS）法测定还原糖含量的适宜条件,建立五味子还原糖的提取及测定方法.方法 以DNS为显色剂,以葡萄糖为对照品,考察测定波长对还原糖测定过程中各因素的影响;比较不同五味子饮片中水提法还原糖的含量.结果 以葡萄糖为对照的适宜波长为530nm,五味子饮片中还原糖含量有一定差别.结论 本法简便、准确、可靠,可用于五味子还原糖的含量测定.
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| 关 键 词： | DNS法 还原糖 适宜条件 五味子 |
Optimum condition for determining reducing sugar content in Schisandra by DNS method |
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| JIANG Jian-li.Optimum condition for determining reducing sugar content in Schisandra by DNS method[J].Strait Pharmaceutical Journal,2014,26(1):57-60. |
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| Authors: | JIANG Jian-li |
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| Institution: | JIANG Jian-li(Fujian Nanping Institute of Drug ControI,Nanping 353000,China) |
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| Abstract: | OBJECTIVE To investigate the optimum condition for determining the content of reducing sugar by DNS Method and establish the extraction and determination method for reducing sugar in Schisandra. METHODS Measurement wavelength was investigated on the influence of various factors in the process of determining reducing sugar with DNS as chromomeric agent and Glucose as reference. Contents of reducing sugar in different Schisandra slices prepared by water extracting were compared. RESULTS The optimum wavelength was 530nm with Glucose as reference. The content of reducing sugar was different in Schisandra slices. CONCLUSION The method is accu- rate, reliable and can be used for determining the content of reducing sugar in Schisandra. |
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| Keywords: | DNS Method Reducing sugar Optimum condition Schisandra |
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