| Isolation of cardiac conduction system of rat heart by laser capture microdissection |
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| 作者姓名： | 欧妍 牛小麟 任付先 黄辰 李喆 陈伟 |
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| 作者单位： | [1]Department of Cardiology, Second Hospital of Xi＇an Jiaotong University, Xi＇an 710004,China [2]Environment and Genes related Diseases Key Laboratory of Education Ministry, Xi＇an 710061 ,China [3]Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth Military Medical University,Xi＇an 710032,China |
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| 基金项目： | Supported by Doctor Grants from Ministry of Education （20030698009） |
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| 摘 要： | Objective：To isolate ceils of cardiac conduction system （CCS） with laser capture microdissec tion （LCM） and extract and evaluate quality of small amount of RNA from ceils of CCS. Methods： Cryo star sections were followed by H-E staining. 20 pieces of H-E stained eryostat sections were scraped and its RNA was assessed to insure that RNA didn＇t degrade in dyeing and dehydration process. Ceils of CCS were captured with LCM and quality of small amount of RNA was verified with RT-PCR. Results： Ceils of CCS isolated with LCM had clear morphology after staining. High quality RNA was extracted from LCM samples and scraped tissues; 18S rRNA and 28S rRNA were seen distinctly on gel eleetrophoresis. Low level of small amount of RNA extracted from LCM sample was below the limit of detection on gel eleetrophoresis or ultraviolet speetrophotometer. The housekeeping genes β-aetin and GAPDH were successfully amplified with small amount of RNA. Conclusion ：This study resolves the problem of acquiring material of CCS precisely that hinders gene research of CCS. It is found out that the method is easy and reliable to extract and assess the quality of small amount of RNA from mierodisseeted ceils of CCS.
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| 关 键 词： | 心脏传导系统 心脏疾病 激光 显微检查 |
| 收稿时间： | 2005-09-19 |
| 修稿时间： | 2005-11-03 |
Isolation of cardiac conduction system of rat heart by laser capture microdissection |
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| OU Yan,NIU Xiao-lin ,REN Fu-xian,HUANG Chen,LEI Cong,LI Zhe,CHEN Wei.Isolation of cardiac conduction system of rat heart by laser capture microdissection[J].Journal of Medical Colleges of PLA(China),2005,20(6):327-332. |
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| Authors: | OU Yan NIU Xiao-lin REN Fu-xian HUANG Chen LEI Cong LI Zhe CHEN Wei |
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| Institution: | OU Yan1,NIU Xiao-lin 1**,REN Fu-xian1,HUANG Chen2,LEI Cong1,LI Zhe1,CHEN Wei3 1Department of Cardiology,Second Hospital of Xi'an Jiaotong University,Xi'an 710004,China 2Environment and Genes related Diseases Key Laboratory of Education Ministry,Xi'an 710061,China 3Department of Oral and Maxillofacial Surgery,Stomatological College,Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | |
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| Keywords: | laser capture microdissection cardiac conduction system RNA RT-PCR |
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