miRNA-101在胃癌组织和细胞中的表达及对胃癌生物学行为的影响

目的:探讨miRNA-101在胃癌组织和细胞中的表达情况及对胃癌细胞生物学行为的影响.方法:应用实时荧光定量PCR法分析31例胃癌组织和癌旁组织样本以及人胃癌细胞系SGC-7901、MKN45、人正常胃黏膜上皮细胞系GES1中miRNA-101的表达水平;建立miRNA-101重组腺病毒载体感染胃癌细胞系SGC-7901、MKN45,对照组为感染阴性对照病毒的胃癌细胞系SGC-7901、MKN45,MTT法检测miRNA-101对胃癌细胞增殖能力的影响;Transwell小室法检测胃癌细胞的迁移和侵袭能力.结果:胃癌组织中miR-101表达水平明显低于癌旁组织(P<0.01);miR-101在细胞系SGC-7901和MKN45中的表达量明显低于人正常胃黏膜上皮细胞系GES1(P<0.01).孵育48 h后MKN45细胞miRNA-101组的细胞增殖能力低于其对照组(P<0.05);而孵育72 h后SGC-7901细胞和MKN45细胞的miRNA-101组细胞增殖能力均亦低于相应对照组(P<0.05).迁移试验结果显示,miRNA-101组迁移的胃癌细胞SGC-7901和MKN45的平均细胞数明显低于相应对照组(P<0.01);侵袭实验结果显示,miRNA-101组侵袭的胃癌细胞SGC-7901和MKN45的平均细胞数亦明显低于其对照组(P<0.01).结论:miRNA-101在胃癌组织和胃癌细胞系中表达下调,转染miRNA-101可明显降低SGC-7901和MKN45细胞的增殖、迁移、侵袭能力,miRNA-101可能参与了胃癌疾病的发生及发展.

The expression level of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer

Objective:To explore the expression levels of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer cells. Methods:The expression levels of miRNA-101 in 31 gastric carcinoma tissue and paraneoplastic tissue samples,human gastric carcinoma cell line SGC-7901 and MKN45,and normal human gastric epithelial cell line GES1 were detected using real-time PCR method. The recombinant adenovirus vector containing miRNA-101 was established,and transfected into the gastric cancer cell line SGC-7901 and MKN45. The effect of miRNA-101 on gastric cancer cell proliferation was detected by MTT method,and the migration and invasion of gastric cancer cells were detected by Transwell method. Results:The expression level of miRNA-101 in gastric cancer tissue was significantly lower than that in adjacent tissue(P<0. 01). The expression levels of miRNA-101 in SGC-7901 and MKN45 cell lines were significantly lower than that in GES1 cells(P<0. 01). The proliferation of MKN45 cells in miRNA-101 group after 48 h of incubation was significantly lower than that in control group(P<0. 05). The proliferations of SGC-7901 and MKN45 cells in miRNA-101 group after 72 h of incubation were lower than that in control group(P<0. 05). The migration test results showed that the average migrated number of SGC-7901 and MKN45 cells in miRNA-101 group were significantly lower than that in control group(P<0. 01). The invasion test results showed that the average invaded number of SGC-7901 and MKN45 cells in miRNA-101 group were significantly lower than that in control group(P <0. 01). Conclusions:The expression levels of miRNA-101 in gastric cancer tissue and gastric cancer cell lines downregulate, the transfection of miRNA-101 can significantly decrease the abilities of proliferation,migration and invasion of SGC-7901 and MKN45 cells. The miRNA-101 may be involved in the occurrence and development of gastric diseases.