石莼、网地藻多糖含量与藻际微生物丰度关系研究

目的：研究石莼、网地藻藻际微生物丰度对多糖含量的影响。方法：通过实时荧光定量PCR(Real-time PCR)技术对石莼、网地藻所附着的藻际微生物丰度进行研究(细菌16S rDNA以及真菌18S rDNA丰度)。结果：石莼多糖含量、藻际微生物总DNA含量均显著高于网地藻(p <0.05)。石莼藻际微生物丰度(细菌1.04×1010-1.42×1010拷贝数/g干样,真菌1.01×107-1.38×107拷贝数/g干样) 显著高于网地藻(细菌2.05×108-6.9×108拷贝数/g干样、真菌2.59×105-4.79×105拷贝数/g干样)(p<0.01)。典型对应分析(CCA分析)表明石莼、网地藻多糖含量与藻际微生物(细菌、真菌)丰度显著相关。结论：石莼、网地藻藻际微生物丰度对多糖含量有一定影响。

Study on the relationship between polysaccharide extraction and algae microorganisms abundance of Ulva lactuca L and Dictyota dichotoma

To observe the characteristics and abundance of microbial communities in different ulva can help us monitor the potential polysaccharide extraction and microbial factors affecting the health of Ulva lactuca L and Dictyota dichotoma. Method: A real-time quantitative PCR (polymerase chain reaction) (RTQ-PCR) method is established to quantitatively detect the abundance of microbial abundance. Results: the polysaccharide extraction yield, total DNA content of the Ulva lactuca L were significantly higher than the Dictyota dichotoma (p < 0.05). The results show that the determination ranges of total bacteria and fungi are 1.04×1010-1.42×1010gene copies /g dry sample and 1.01×107-1.38×107 gene copies /g dry sample of Ulva lactuca L . The abundance of Ulva lactuca L was significantly higher than Dictyota dichotoma (bacteria: 2.05×108-6.9×108 gene copies /g dry; fungi: 2.59×105-4.79×105gene copies /g dry) (p < 0.01).Analysis of typical correspondence analysis (CCA) showed that polysaccharide from Ulva lactuca L and Dictyota dichotoma were significantly associated with microbial abundance. Conclution: The abundance of microbial communities have certain influence to polysaccharide extraction.