β-细辛醚对抑郁模型大鼠行为及生物钟基因表达的影响

目的:探讨β-细辛醚对抑郁模型大鼠生物钟基因(clock)表达的影响。方法:将雄性SD大鼠随机分为正常组,模型组,agomelatine(褪黑素药物激动剂)组,β-细辛醚低、高剂量组5组,每组10只。给予28 d慢性不可预见性刺激(CMUS),复制大鼠抑郁模型。第8天开始模型组、agomelatnie组、β-细辛醚组分别给予生理盐水,agomelatnie(40 mg·kg-1·d-1),β-细辛醚低、高剂量组(12.5,25 mg·kg-1·d-1),5 m L·kg-1,ig。于给药前及第28天分别检测大鼠体重、水平活动次数及糖水偏爱度,评价大鼠行为学改变。处死大鼠冰上取脑,利用实时PCR及,Western blot方法,检测生物钟基因在各组大鼠脑中表达。结果:在行为学方面,模型组大鼠28 d后体重的增加程度,水平活动次数及糖水偏爱度均显著低于正常组及给药组。在基因表达方面,与正常组相比较,模型组clock的表达显著高于正常组,agomelatnin组,β-细辛醚2个剂量组clock的表达无显著差别;与模型组相比较,agomelatine组,β-细辛醚两剂量组clock的表达显著低于模型组。结论:β-细辛醚可能通过影响抑郁大鼠生物钟基因clock在脑中的表达改变大鼠的抑郁状态。

Effect of Beta-asarone on Behaviors and Expression of Circadian Genes in Rat Model of Depression

Objective: To investigate the effect of beta-asarone on the expression of circadian gene clock in depression model rats. Method: Male SD rats were randomly divided into five groups:normal group, model group, agomelatine (melatonin agonist group drug), beta-asarone low-dose group, high-dose group with 10 rats in each group,chronic unpredictable stimulation (CMUS) was given for 28 days to copy the rat model of depression. From the beginning of the eighth day model group, agomelatine group, beta-asarone low-dose groups, beta-asarone high-dose group were given normal saline, agomelatin(40 mg · kg^-1 · d^-1), beta-asarone(12.5,25 mg · kg^-1 ·d^-1), 5 mL · kg^-1 gavage. The body weight,horizontal movement and sucrose preference were detected for assessing rat ethological changes on the beginning of the experiment and the 28^th day. Then they were sacrificed and, taken the rats brain out on the ice. The expression of circadian gene were detected by using Q-PCR, Western blotting. Result: On behaviors,after 28 days the increases of the model rat body weight were significantly lower than normal group and drug group.On expression of the circadian gene clock, compared with normal group, the expression of circadian gene clock in model group was significantly higher than that in normal group. There was no significant difference among the agomelatine group, beta-asarone two doses group, and the normal group on the expression of the circadian gene clock;compared with the model group, the expression of the gene in agomelatonin group, beta-asarone two doses group were significantly lower than the model group;compared with agomelatine group, no significant difference in the expression of clock among the beta-asarone low dose group, the beta-asarone high dose group and the agomelatine group. Conclusion: beta-asarone may improve the depressive state in depression by affecting the expression of the depression in circadian clock genes clock rats.