| 血链球菌细菌素对唾液生物膜中牙龈卟啉单胞菌和具核梭杆菌拮抗作用的实验研究 |
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| 引用本文： | 马晟利,李旭明,王琪波,李海清,李春梅. 血链球菌细菌素对唾液生物膜中牙龈卟啉单胞菌和具核梭杆菌拮抗作用的实验研究[J]. 口腔医学研究, 2011, 27(5): 380-382 |
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| 作者姓名： | 马晟利 李旭明 王琪波 李海清 李春梅 |
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| 作者单位： | 1. 黑龙江省医院口腔科,黑龙江,哈尔滨,150036
2. 佳木斯大学 |
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| 基金项目： | 黑龙江省自然科学基金资助项目 |
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| 摘 要： | 目的:提取血链球菌标准菌株(ATCC10556)细菌素,研究其对唾液生物膜中牙龈卟啉单胞菌和具核梭杆菌的拮抗作用.方法:通过超声破碎,高速离心,盐析等方法提取血链球菌细菌素;采用二倍稀释法,测定血链球菌细菌素对浮游状态下牙龈卟啉单胞菌和具核梭杆菌的最小抑制浓度(MIC);在体外建立牙龈卟啉单胞菌和具核梭杆菌唾液生物膜模...
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| 关 键 词： | 血链球菌 细菌素 拮抗 牙龈卟啉单胞菌 具核梭杆菌 |
Study of Antagonistic Effects of Sanguicin on Salivary Biofilm of Porphyromonas Gingivalis and Fusobacterium Nucleatum in Vitro |
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| MA Sheng-li,LI Xu-ming,WANG Qi-bo,et al.. Study of Antagonistic Effects of Sanguicin on Salivary Biofilm of Porphyromonas Gingivalis and Fusobacterium Nucleatum in Vitro[J]. Journal of Oral Science Research, 2011, 27(5): 380-382 |
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| Authors: | MA Sheng-li LI Xu-ming WANG Qi-bo et al. |
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| Affiliation: | MA Sheng-li,LI Xu-ming,WANG Qi-bo,et al.Department of Stomatology Hospital of Heilongjiang Province,Harbin 150036 |
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| Abstract: | Objective:To investigate the effects of sanguicin extracted from streptococcus sanguis on salivary biofilm of Porphyromonas gingivalis(P.g) and Fusobacterium nucleatum(F.n) in vitro.Methods:Ultrasonic,centrifugation and ammonium sulfate were used to extract sanguicin.The minimal inhibitory concentration(MIC) of sanguicin against P.g and F.n was measured by the broth dilution technique.Biofilms formed by P.g and F.n were observed by confocal laser scan microscope(CLSM) to determine the percentage of vital ce... |
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| Keywords: | Streptococcus.s Sanguicin Antagonize P.g F.n |
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