高锗灵芝对自然衰老大鼠血清超氧化物歧化酶活性及丙二醛含量的影响

目的探讨高锗灵芝对自然衰老大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法40只自然衰老大鼠随机分成对照组、高锗灵芝低剂量组、中剂量组和高剂量组。将高锗灵芝粉剂配成浓度分别为20 g.L-1、40 g.L-1和80 g.L-1的溶液,分别灌胃低剂量组、中剂量组和高剂量组的大鼠,对照组用蒸馏水灌胃,每只大鼠灌胃的溶液体积为10 mL.kg-1。每日1次,45 d后断头处死,取血,测定血清SOD、MDA的含量。结果高锗灵芝中、高剂量组大鼠血清SOD含量均显著高于对照组和低剂量组(P<0.05);MDA含量均显著低于对照组和低剂量组(P<0.05),中剂量组与高剂量组之间及对照组与低剂量组之间血清SOD、MDA含量均无显著差异(P>0.05)。结论服用高锗灵芝的量应适中。中、高剂量的高锗灵芝可减少自然衰老大鼠血清自由基的产生,加速自由基的清除,具有一定抗衰老的生物学作用。

Effect of high germanium ganoderma on the activity of serum superoxide dismutase and malondialdehyde of natural senescent rats

Objective To investigate the effect of high germanium ganoderma on the activity of serum superoxide dismutase（SOD） and malondialdehyde（MDA） of natural senescent rats. Methods Forty natural senescent rats were randomly divided into four groups ： control group, low, middle and high doses of high germanium ganoderma group. High germanium ganoderma power was diluted to the concentration of 20 g ·L^-1,40 g ·L^-1 and 80 g·L^-1, then the low, middle and high doses of high germanium ganoderma group were given the different concentration solution with intragastric administration respectively, and the control group was given distilled water. The solution volume of all rats with intragastric administration was 10 mL ·kg^-1 ,once a day,then killed by breaking head,and collected the blood to determine the content of SOD and MAD. Results The contents of SOD in middle and high doses of high germanium ganoderma group were significantly higher than those in control group and low doses of high germanium ganoderma group （ P 〈 0.05 ）, and the contents of MDA were significantly lower than those in control group and low doses of high germanium ganoderma group（P 〈 0.05）. There is no significant difference of those between middle doses of high germanium ganoderma group and high doses of high germanium ganoderma group and between control group and low doses of high germanium ganoderma group （ P 〈 0. 05）. Conclusion The dose of high germanium ganoderma should be taken moderately. Middle and high doses of high germanium ganoderma can reduce the free radical and accelerate the clearance of the free radical. It has certain anti-aging effect.