| 异甘草素对豚鼠心室肌细胞L型钙通道的影响 |
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| 引用本文： | 冯斯婷,唐其柱,易方方,罗浩,王腾.异甘草素对豚鼠心室肌细胞L型钙通道的影响[J].武汉大学学报(医学版),2006,27(1):28-31. |
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| 作者姓名： | 冯斯婷 唐其柱 易方方 罗浩 王腾 |
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| 作者单位： | 武汉大学人民医院心内科,武汉,430060 |
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| 摘 要： | 目的:研究异甘草素对豚鼠单个心室肌细胞膜ICa-L通道的影响,旨在探讨其抗心律失常的机制。方法:采用全细胞膜片钳技术,观察不同浓度的异甘草素对ICa-L的作用。结果:①异甘草素能使I-V曲线明显上移。②异甘草素0.1-100μmol/L对ICa-L呈浓度依赖性的抑制作用。③3μmol/L异甘草素具有频率依赖性的抑制作用,它能使ICa-L稳态激活曲线右移、失活曲线左移,还能增加失活后恢复的τ值。结论:提示异甘草素对ICa-L通道的激活态和失活态有较高亲和力,这些可能成为其抗心律失常作用的机制。
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| 关 键 词： | 异甘草素 L型钙电流 全细胞膜片钳 心室 豚鼠 |
| 文章编号： | 1671-8852(2006)01-0028-04 |
| 收稿时间： | 2005-08-25 |
| 修稿时间： | 2005年8月25日 |
Inhibitory Effect of Isoliguiritigenin on L-type Calcium Currents in Ventricular Myocytes of Guinea Pigs |
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| FENG Siting,TANG Qizhu,YI Fangfang,LUO Hao,WANG Teng.Inhibitory Effect of Isoliguiritigenin on L-type Calcium Currents in Ventricular Myocytes of Guinea Pigs[J].Medical Journal of Wuhan University,2006,27(1):28-31. |
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| Authors: | FENG Siting TANG Qizhu YI Fangfang LUO Hao WANG Teng |
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| Institution: | Dept. of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China |
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| Abstract: | Objective: To study the effects of Isoliguiritigenin(ISO) on ICa-L of ventricular myocytes in guinea pigs.Methods: Standard whole-cell patch clamp technique was used.Results: ① ISO could make the I-V curves upward.② It reduced ICa-L in a concentration-dependent manner at the range of 0.1-100 μmol/L.③ ISO in the concentration of 3 μmol/L had a frequency-dependent blocking function,and made the steady-state activation curves shift to right,inactivation curve to left,and the τ value of recovery duration increase obviously.Conclusion: ISO exerts direct blocking effect by a strong affinity with activated states and inactivated states. |
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| Keywords: | Isoliguiritigenin L-type Calcium Currents Patch Clamp Ventrium Guinea Pig |
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