NO对兔局灶脑缺血脑水肿和脑梗塞灶的影响

本文在兔MCAO局灶脑缺血模型基础上,利用外源性一氧化氮(NO)前体物质L-精氨酸和NOS抑制剂L-NNA研究NO对脑缺血后脑水肿和脑梗塞的影响。NZW兔42只随机分为7组,每组6只,计对照组、假手术组、MCAO组、MCAO+NS组、MCAO+L-Arg组、MCAO+D-Arg组及MCAO+L-Arg+L-NNA组。结果提示:与单纯MCAO组比较,L-Arg组的脑组织H_2O、Na~+、Ca~(2+)含量明显下降(P<0.01),脑梗塞灶亦明显缩小(P<0.01)。从而显示L-Arg系通过产生NO促使血管舒张而减轻脑水肿和缩小梗塞灶,为临床改进脑缺血的治疗提供依据。

The Influence of Nitric Oxide (NO) on the Brain Edema and Infarction after Focal Brain Ischemia in the Rabbits

Based on rabbit MCAO focal brain ischemic model, we studied the effect of NO on ischemic brain by using L-Arginine, a precursor of NO and NOS inhibitor L-NNA. 42 NZW rabhits were divided into 7 groups, namely control group, sham-operated group, MCAO ischemic group, MCAO + NS group, MCAO + L-Arg group, MCAO + D-Arg group, MCAO +L-Arg+L-NNA group with 6 each. The results verified that the brain tissue H2O, Na+, Ca2+ contents were decreased significantly (P<0. 01) in L-Arg treated group compared with simple ischemic MCAO group. The brain infarction volume was also decreased remarkably (P<0.01). It is suggested that the effect of L-Arg was accomplished through the production of NO. NO has the function of relaxing blood vessels, hence lessening ischemic brain edema and decreasing the infarct volume, which may improve the clinical management of brain ischemia.