Method for primary culture of purified leydig cells isolated from adult rat testes |
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| Authors: | Joan Y. Browning Jerrold J. Heindel H. Edward Grotjan Jr. |
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| Affiliation: | (1) Department of Reproductive Medicine and Biology, University of Texas Health Science Center Medical School, P.O. Box 20708, 77025 Houston, Texas, USA |
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| Abstract: | ![]()
Summary Preparations of 75–80% pure Leydig cells are prepared by Percoll gradient centrifugation of crude testicular interstitial cell suspensions. Using the methods described herein, the purified Leydig cells retain their functional characteristics for at least 72 hours in primary culture. |
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| Keywords: | Leydig cells Percoll gradients testicular interstitial primary cell culture |
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