基于调控大鼠胃肠c-kit和SCF mRNA表达的枳壳燥性及炮制减燥机制分析

目的:明确枳壳燥性的产生机制,并比较不同炮制品对正常大鼠胃窦、结肠c-kit和干细胞因子(SCF)mRNA表达的影响。方法:将SD大鼠分为空白组,生品枳壳高(10 g·kg~(-1),下同)和低(1 g·kg~(-1),下同)剂量组,麸炒枳壳高、低剂量组,蜜麸枳壳高、低剂量组及慢传输型便秘(STC)模型组,各给药组大鼠灌服相应药液1次/d,模型组大鼠灌服复方地芬诺酯片溶液,空白组大鼠灌服同等体积生理盐水,所有实验动物均连续给药30 d。在第30 d处死大鼠并迅速剖取胃窦与结肠组织,采用实时荧光定量聚合酶链式反应扩增仪检测组织中c-kit和SCF mRNA的表达水平。结果:与空白组比较,生品枳壳组胃窦与结肠的c-kit和SCF mRNA表达均显著降低(P0.01)。与模型组比较,生品枳壳高剂量组所有检测指标均无显著性差异;麸炒枳壳组、蜜麸枳壳组胃窦与结肠的c-kit和SCF mRNA表达有显著增加(P0.05,P0.01)。结论:长期高剂量服用枳壳生品能显著抑制正常大鼠胃肠道c-kit和SCF mRNA的表达,进而影响胃肠道Cajal间质细胞的数量和功能,从而造成肠燥便秘;麸炒枳壳与蜜麸枳壳均能有效缓和生品所产生的肠道燥性效应,且蜜麸炒品炮制减燥作用优于麸炒品。

Analysis of Dryness Nature and Processing Mechanism for Reducing Dryness of Aurantii Fructus by Regulating Expression of c-kit and SCF mRNA in Gastrointestinal Tract of Rats

Objective: The mechanism of dryness nature of Aurantii Fructus was clarified, and the effects of different processed products on c-kit and stem cell factor(SCF) mRNA expression of sinuses ventriculi and colon in normal rats were compared. Method: SD rats were divided into blank group, high(10 g·kg^-1, similarly hereinafter) and low(1 g·kg^-1, similarly hereinafter) dose group of crude Aurantii Fructus, high and low dose group of Aurantii Fructus processed with bran, high and low dose group of Aurantii Fructus processed with honey bran and slow transit constipation(STC) model group.Rats in each medication administration group were given corresponding solution with 1 time for a day, rats in model group were given compound diphenoxylate tablets solution and rats in the blank group were given the same volume of normal saline.All the experimental animal were administered continuously for 30 days.All rats were executed on the 30^th day, and the sinuses ventriculi and colon tissues were immediately dissected.After that, the expression levels of c-kit and SCF mRNA in tissues were detected by real-time fluorescence quantitative polymerase chain reaction(PCR) amplifier. Result: Compared with the blank group, the c-kit and SCF mRNA expression of sinuses ventriculi and colon in crude Aurantii Fructus group were significantly decreased(P<0.01).Compared with the model group, there was no significant difference in all indexes of crude Aurantii Fructus group with high dose;and all the indexes of the other two groups were increased significantly(P<0.05, P<0.01). Conclusion: Keeping use high-dose of crude Aurantii Fructus in a long-term can significantly reduce the expression of c-kit and SCF mRNA in normal rat gastrointestinal tract, thus affecting the number and function of interstitial cells of Cajal in gastrointestinal tract, and resulting in intestinal dryness and constipation.Both of Aurantii Fructus processed with bran and honey bran can effectively alleviate the intestinal dryness effect caused by crude Aurantii Fructus, and Aurantii Fructus processed with honey bran has the better effect on reducing dryness than bran products.