基于谱效关系的朝鲜天南星和东北天南星相似性评价

目的:对天南星药材指纹图谱与抗肿瘤药效进行相关分析,探讨基于主要药效成分的朝鲜天南星与东北天南星化学成分相似性,为朝鲜天南星临床合理应用提供实验数据。方法:采用高效液相色谱法,建立11批不同产地、不同采收期朝鲜天南星与东北天南星药材指纹图谱;采用噻唑蓝(MTT)比色法考察其对人肝癌SMMC-7721细胞的增殖抑制作用;同时采用灰色关联度分析、偏最小二乘回归分析方法,对6批朝鲜天南星和5批东北天南星进行谱效相关性分析,找到各自药效贡献较大成分,评价二者相似性。结果:成功建立了11批不同来源天南星药材的指纹图谱,同时验证了不同来源天南星的抗肿瘤药理活性。灰色关联分析结果显示朝鲜天南星与药效关联系数0.80的15个成分中有12个与东北天南星一致;偏最小二乘回归分析验证了灰色关联分析结果的合理性。综合分析证明,基于主要药效成分的朝鲜天南星与东北天南星具有较好的相似性。结论:本实验以抗肿瘤药效为导向,验证了朝鲜天南星与东北天南星基于化学成分的相似性,为朝鲜天南星作为习用品提供合理依据的同时,也为其质量控制提供实验依据。

Similarity Evaluation of Arisaema peninsulae and Arisaema amurense Based on Spectrum-activity Relationship

Objective: Fingerprint and anti-tumor pharmacodynamic analysis were used to probe the similarity of the principal pharmacodynamic components of Arisaema peninsulae and A. amurense,providing data support for the rational clinical use of A. peninsulae. Method: High performance liquid chromatography (HPLC) method was used to establish the fingerprint of 11 batches of A. peninsulae and A. amurense from different origins or harvest time points. Thiazolyl blue(MTT) assay was used to investigate their inhibition effect on SMMC-7721 cells proliferation. Grey relational analysis and partial least-squares regression analysis methods were used to analyze the spectrum-activity relationship of 6 batches of A. peninsulae and 5 batches of A. amurense respectively, aimed to find the ingredients with greater contribution to efficacy and evaluate the similarities. Result: The fingerprint of 11 batches of A. peninsulae and A. amurense from different origins or harvest time points was established, and their anti-tumor activity was verified. Grey relational analysis results showed that 12 components from 15 components of A. peninsulae whose pharmacodynamic correlation coefficient was greater than 0.80 were the same as A. amurense. And the rationality of the grey relational analysis results was verified by partial least-squares regression analysis. The comprehensive analysis proved that A. peninsulae had a good similarity with A. amurense based on the main pharmacodynamic components. Conclusion: Guided by the anti-tumor efficacy,this experiment verified the similarity of chemical compositions between A. peninsulae and A. amurense,providing reasonable basis for the use of A. peninsulae,and providing experimental basis for its quality control.