| HPLC-MS鉴定塔中栽培肉苁蓉药材的17个成分 |
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| 引用本文： | 南泽东,任华忠,赵明波,姜勇,屠鹏飞. HPLC-MS鉴定塔中栽培肉苁蓉药材的17个成分[J]. 中国实验方剂学杂志, 2018, 24(20): 62-67 |
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| 作者姓名： | 南泽东 任华忠 赵明波 姜勇 屠鹏飞 |
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| 作者单位： | 乐山职业技术学院;北京大学天然药物及仿生药物国家重点实验室 |
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| 基金项目： | 国家"重大新药创制"科技重大专项（2012ZX09201201-001,2012ZX09301002-002-002,2012ZX09304-005） |
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| 摘 要： | 目的:通过HPLC-MS对新疆塔中荒漠栽培肉苁蓉Cistanche deserticola药材60%甲醇提取物的色谱图进行鉴定。方法:采用Agilent ZORBAX Eclipse Plus C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(含0.1%的甲酸)为流动相进行梯度洗脱,流速1 mL·min~(-1),检测波长254 nm,柱温25℃,进样量10μL。结果:在相同色谱条件下,通过与已分离得到的对照品的保留时间、相对分子质量以及碎片离子进行比较,对新疆塔中荒漠栽培肉苁蓉药材的16个色谱峰中的17个成分进行了准确的鉴定,分别确定为松柏苷,去甲紫丁香苷,紫丁香苷,松果菊苷,肉苁蓉苷A,类叶升麻苷(管花苷A),肉苁蓉苷B,异类叶升麻苷,2'-乙酰基肉苁蓉苷A,肉苁蓉苷C,异肉苁蓉苷C,2'-乙酰基类叶升麻苷,管花苷B,epimeridinoside A,肉苁蓉苷K,肉苁蓉苷J。结论:该研究方法适用于肉苁蓉60%甲醇提取物色谱峰的鉴定,为全面分析该药材的化学成分提供了一定的科学数据。
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| 关 键 词： | 荒漠肉苁蓉 肉苁蓉属 液质联用 甲醇提取物 |
| 收稿时间： | 2017-12-08 |
Identification of Seventeen Components in Cistanches Herba Cultured in Tarim Desert by HPLC-MS |
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| NAN Ze-dong,REN Hua-zhong,ZHAO Ming-bo,JIANG Yong and TU Peng-fei. Identification of Seventeen Components in Cistanches Herba Cultured in Tarim Desert by HPLC-MS[J]. China Journal of Experimental Traditional Medical Formulae, 2018, 24(20): 62-67 |
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| Authors: | NAN Ze-dong REN Hua-zhong ZHAO Ming-bo JIANG Yong TU Peng-fei |
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| Affiliation: | Leshan Vocational and Technical College, Leshan 614000, China,Leshan Vocational and Technical College, Leshan 614000, China,State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China,State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China |
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| Abstract: | |
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| Keywords: | Cistanches Herba Cistanche HPLC-MS methanol extract |
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