金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响

目的:探讨金龙胶囊对人胃癌MGC-803,BGC-823细胞侵袭和迁移能力的影响并对其具体机制进行探讨。方法:体外培养胃癌MGC-803,BGC-823细胞,分为金龙胶囊组(0.1,0.2,0.4,0.8 g·L~(-1)),空白组和5-氟尿嘧啶(5-FU)组,采用噻唑蓝(MTT)比色法分别检测金龙胶囊对MGC-803,BGC-823细胞增殖的抑制作用,计算半数抑制率(IC50);采用细胞侵袭(transwell)小室法和划痕实验检测金龙胶囊处理MGC-803和BGC-823细胞24 h后,细胞侵袭和迁移能力发生改变;蛋白免疫印迹法(Western blot)检测金龙胶囊处理MGC-803,BGC-823细胞24 h后E-钙黏素(E-cadherin),基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)蛋白表达情况。结果:金龙胶囊能明显抑制MGC-803,BGC-823细胞的增殖,呈一定的浓度及时间依赖性;与空白组比较,金龙胶囊能明细减少MGC-803,BGC-823细胞的穿膜细胞数目,可明显影响2种胃癌细胞的侵袭与迁移能力,并呈明显浓度依赖性(P0.05);金龙胶囊组的划痕距离较空白组明显降低,影响MGC-803,BGC-823细胞迁移,并呈浓度依赖性(P0.05);与空白组比较,金龙胶囊组能够提高MGC-803和BGC-823细胞中E-cadherin蛋白表达,降低MMP-2,MMP-9蛋白表达水平,且呈一定的量效关系(P0.05)。结论:金龙胶囊能够抑制人胃癌MGC-803,BGC-823细胞的侵袭和迁移能力,其作用机制可能与上调E-cadherin表达,抑制MMP-2,MMP-9表达水平有关。本研究证实了金龙胶囊对胃癌MGC-803,BGC-823细胞抗侵袭转移的部分机制。

Effect of Jinlong Capsule on Invasion and Migration of Human Gastric Carcinoma MGC-803 and BGC-823 Cells

Objective: To investigate the effect of Jinlong capsule(JLC) on the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells and its specific mechanism. Method: Gastric cancer MGC-803 and BGC-823 cells were cultured in vitro and divided into JLC group (with concentrations of 0.1, 0.2, 0.4, 0.8 g·L^-1), negative control group and 5-fluorouracil (5FU) as positive control group. Methye thiazolye telrazlium (MTT) was used to detect the inhibitory effect of Jinlong capsule on the proliferation inhibition rate of MGC-803 and BGC-823 cells, in order to calculate the 50%inhibitory concentration (IC₅₀); transwell and wound healing assay were used to detect the invasion and migration abilities of BGC-823 cell and MGC-803 cells after treatment with JLC for 24 h; after 24 h, the migration of MGC-803 and BGC-823 cells changed; Western blot method was used to detect the expression levels of E-cadherin, matrix metalloproteinase (MMP)-2 and MMP-9 after Jinlong capsule treatment for 24 h. Result: JLC could obviously inhibit the proliferation of MGC-803 and BGC-823 cells in a time-dependent manner; compared with the control group, JLC could significantly affect the invasion and migration abilities of the two kinds of gastric cancer cells in a concentration-dependent manner (P<0.05); the scratch distance of Jinlong capsule group was significantly lower than that in control group in a concentration-dependent manner (P<0.05); compared with control group, JLC group could increase the expression of E-cadherin and reduce the expression levels of MMP-2, MMP-9 in MGC-803 and BGC-823 cells, with a dose-response relationship (P<0.05). Conclusion: JLC can inhibit the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells, and its mechanism may be related to the up-regulation of the expression of E-cadherin and the inhibition of the expression levels of MMP-2, MMP-9.This study has confirmed part of mechanisms of JLC on the invasion and metastasis of gastric cancer MGC-803 and BGC-823 cells.