肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响

目的:观察肉苁蓉不同提取部位对氢化可的松致肾阳虚模型大鼠性能力的影响。方法:将50只雄性SD大鼠随机分为正常组、阳虚模型组、肉苁蓉总寡糖组、肉苁蓉总多糖组、肉苁蓉苯乙醇总苷组。连续14 d肌肉注射氢化可的松制备肾阳虚动物模型,给药组按肉苁蓉生药量2.3 g·kg-1对应给予肉苁蓉总寡糖103.82 mg·kg-1,肉苁蓉总多糖115.64 mg·kg-1,肉苁蓉苯乙醇总苷201.48 mg·kg-1水溶液,每日1次。14 d后测定各组大鼠的勃起潜伏期,交配能力各指标(包括捕捉潜伏期、捕捉次数、射精潜伏期、射精次数、捕捉率、射精率等),脏器湿重及精浆果糖含量,血浆环磷酸腺苷(c AMP)/环磷酸乌苷(c GMP)值,血清激素睾酮(T),雌二醇(E2),促卵泡生成素(FSH),促黄体生成素(LH)及促性腺激素释放激素(GnRH)水平,观察睾丸病理形态变化。结果:肉苁蓉不同提取部位对肾阳虚大鼠的勃起功能、交配能力、脏器湿重及精浆果糖含量,血浆c AMP/c GMP值,血清激素水平,睾丸病理形态变化均有作用。其中肉苁蓉苯乙醇总苷提取物能够显著缩短大鼠的勃起潜伏期(P0.05)及捕捉潜伏期(P0.01),显著增加捕捉次数(P0.01),睾丸(P0.05),精囊腺(P0.01)湿重及精浆果糖含量(P0.05),显著升高血浆c AMP/c GMP值(P0.01)及血清T(P0.01),FHS(P0.01),GnRH(P0.05)水平,显著降低血清E2(P0.05),LH(P0.05)水平,显著改善睾丸病理形态。结论:肉苁蓉不同提取部位可通过调控氢化可的松致肾阳虚大鼠模型的激素水平不同程度提高其性能力,其中苯乙醇总苷可显著改善肾阳虚大鼠性能力相关指标。

Effect of Different Fractions of Cistanches Herba Extracting in Improving Sexual Ability of Kidney-Yang Deficiency Rats

Objective:To observe the effect of extracts from on the sexual performance of rats with kidney-Yang deficiency induced by hydrocortisone. Method:Fifty male SD rats were randomly divided into normal control group, kidney-Yang deficiency model group, Cistanches Herba total oligosaccharide group, Cistanches Herba total polysaccharide group, and Cistanches Herba total phenylethanol glycosides, with 10 in each group. The animal model of kidney-Yang deficiency was prepared through intramuscular injection with hydrocortisone for 14 consecutive days. The medication groups were given aqueous solutions of total oligosaccharides of Cistanches Herba(103.28 mg·kg^-1), total polysaccharides of Cistanches Herba (115.64 mg·kg^-1), and total glycosides of phenylethanol of Cistanches Herba (201.48 mg·kg^-1), according to the dose of 2.3 g·kg^-1 of Cistanches Herba raw material once a day. After 14 days, erectile latency, indexes of mating ability (including capture latency, the number of captures, the ejaculation latency, the number of ejaculations), organ wet weight and berry sugar content, plasma cyclic adenosine monophosphate(cAMP)/cyclic guanosine monophosphate(cGMP) ratio, serum hormone testosterone (T), estradiol (E₂), and follicle-stimulating hormone (FSH), luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) levels were determined in each group of rats, and the testicular morphological changes were observed. Result:The extracts from different fractions of Cistanches Herba had the effect on erectile function, mating ability, organ wet weight and berry sugar content, plasma cAMP/cGMP value, serum hormone level, and testicular pathological changes in rats with kidney-Yang deficiency. Particularly, the extracts of total phenylethanol glycosides had the best effect, and could significantly reduce the erectile latency (P<0.05) and capture latency (P<0.01), significantly increase the times of capture (P<0.01), testis (P<0.05), seminal vesicles (P<0.01) wet weight and berry sugar content (P<0.05), significantly increase plasma cAMP/cGMP values (P<0.01), serum T (P<0.01), FHS (P<0.01), GnRH (P<0.05) levels, significantly reduce serum E₂ (P<0.05), LH (P<0.05) levels, and significantly improve pathological morphology of testis in rats. Conclusion:Extracts from different fractions of Cistanches Herba can regulate hormone levels in rats with kidney-Yang deficiency induced by hydrocortisone and further improve their sexual ability. The total glycosides of phenylethanol can significantly improve the indexes related to sexual performance in rats with kidney-Yang deficiency.