| Effects of Placental Isoferritin on the Mouse Embryo Development in vitro |
| |
| 引用本文： | 朱颖,吴超英,孙永玉. Effects of Placental Isoferritin on the Mouse Embryo Development in vitro[J]. 华中科技大学学报(医学英德文版), 2007, 27(5): 579-581. DOI: 10.1007/s11596-007-0526-y |
| |
| 作者姓名： | 朱颖 吴超英 孙永玉 |
| |
| 作者单位： | Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
| |
| 摘 要： | To investigate the effect of placental isoferritin （PLF） on mouse embryo development in vitro, mice 2-cell embryos were co-cultured with human first trimester decidual cells at different concentrations of PLF in vitro. The following changes of the above system were observed under an invert microscope and the number of embryos were recorded and the embryos were classified. The results showed there was no significant difference in the percentage of embryos development to 4-cell 8-cell and morula （P〉0.05）. PLF at the doses of 10 and 100 U/mL significantly enhanced more embryos development to the blastocyst and hatching blastocyst （P〈0.05）. PLF at the dose of 1000 U/mL depressed more embryos development from 2-cell to hatching blastocyst, meanwhile such phenomena as cell degeneration and irregular cleavage were observed in part of embryos, but there was no significant difference in statistics （P〉0.05）. It was concluded that PLF at the concentration of 10-- 100 U/mL had no significant effects on the early development of mice embryos, however, PLF could promote the growth, differentiation, and hatching of preimplantion blastocysts.
|
| 关 键 词： | 胎盘异铁蛋白 胚胎生长 体外试验 动物模型 |
| 收稿时间： | 2006-01-13 |
Effects of placental isoferritin on the mouse embryo development in vitro |
| |
| Zhu Ying,Wu Chaoying,Sun Yongyu. Effects of placental isoferritin on the mouse embryo development in vitro[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2007, 27(5): 579-581. DOI: 10.1007/s11596-007-0526-y |
| |
| Authors: | Zhu Ying Wu Chaoying Sun Yongyu |
| |
| Affiliation: | Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China |
| |
| Abstract: | To investigate the effect of placental isoferritin (PLF) on mouse embryo development in vitro, mice 2-cell embryos were co-cultured with human first trimester decidual cells at different concentrations of PLF in vitro. The following changes of the above system were observed under an invert microscope and the number of embryos were recorded and the embryos were classified. The results showed there was no significant difference in the percentage of embryos development to 4-cell, 8-cell and morula (P>0.05). PLF at the doses of 10 and 100 U/mL significantly enhanced more embryos development to the blastocyst and hatching blastocyst (P<0.05). PLF at the dose of 1000 U/mL depressed more embryos development from 2-cell to hatching blastocyst, meanwhile such phenomena as cell degeneration and irregular cleavage were observed in part of embryos, but there was no significant difference in statistics (P>0.05). It was concluded that PLF at the concentration of 10-100 U/mL had no significant effects on the early development of mice embryos, however, PLF could promote the growth, differentiation, and hatching of preimplantation blastocysts. |
| |
| Keywords: | co-culture fetal development placental isoferritin |
| 本文献已被 维普 万方数据 SpringerLink 等数据库收录！ |
|
