八肽胆囊收缩素对大肠杆菌致急性肺损伤大鼠的抗炎作用

目的 探讨八肽胆囊收缩素(CCK-8)对大肠杆菌致急性肺损伤(ALI)大鼠肺水肿及炎症反应的抑制作用.方法 将45只雄性SD大鼠按随机数字表法分为3组,ALI/急性呼吸窘迫综合征(ARDS)组、ALI/ARDS+ CCK-8组和对照组.ALI/ARDS组气管注射大肠杆菌制作ALI/ARDS模型；ALI/ARDS+ CC...

Inflammation inhibitive effect of CCK-8 on acute lung injury rats induced by Escherichia coli

Objective To explore the inhibitive effects and potential mechanisms of CCK-8 on ALI/ARDS of infected rats induced by E.coli injection.Methods Forty-five SD male rats were randomly divided into control group,ALI/ARDS group,and ALI/ARDS+CCK-8 group.ALI/ARDS model was induced in rats by 0.9％ normal saline E.coli injection.The rats of ALI/ARDS+CCK-8 group was injected by CCK-8 (60 μtg · kg-1 ) 8 h after E.coli injection,and the control group rats were injected by 0.9％ normal saline in trachea.12 h and 24 h after E.coli or 0.9％ normal saline intratracheal injection,PaO2/FiO2 was recorded,and at 24 h after injetion lung wet/dry ratio,microscopic examination and apoptosis of lung tissue were examined,and endothelin 1 (ET-1) was detected by radioimmunoassay.Results Compared with those of ALI/ARDS group,the PaO2/FiO2 was significantly higher (12 h:312±25 vs 249±37,P ＜0.01;24 h:297±39 vs 168±44,P ＜0.001),the lung wet/dry ratio was decreased (4.28 ± 0.23 vs 4.87±0.29,P ＜ 0.05),and alveolar edema and interstitial edema and infiltration of inflammatory cells were unconspicuous under microscope.The apoptosis index of lung tissue and were significantly lower compared with ALI/ARDS group (P ＜ 0.05).The ET-1 was significantly decreased in ALI/ARDS + CCK-8 group ( P ＜ 0.05).Conclusions Taken together the findings indicate that CCK-8 can inhibit the inflammatroy reaction of ALI/ARDS in rats pneumonia,relieve lung edema,protect the lung cells,downregulat the ET-1 and reduce the vascular leakage function,and increase PaO2/FiO2.The CCK-8 can better the ALI/ARDS clinical symptom significantly.