丙泊酚对内毒素性急性肺损伤大鼠肺组织c-Jun氨基末端激酶活性的影响

目的 探讨大鼠内毒素性急性肺损伤(acute lung injury,ALI)时应用丙泊酚后处理对肺组织磷酸化c-Jun氨基末端激酶(phospho-c-Jun terninal kinnase,p-JNK)的影响.方法 96只雄性SD大鼠按数字随机表法分为4组(每组24只):生理盐水对照组(A组)；内毒素[有效成分为...

Propofol attenuates c-Jun terninal kinase activation in lipopolgsaccharides-induced acute lung injury in rats

Objective To investigate the effects of c-Jun terninal kinase(JNK)activation post-trested by propofol on lipopolysaccharide-induced acute lung injury.Methods Ninety six male SD rats were randomly divided into 4 equal groups(n=24):the control group(0.9％ sodium chloride group); Lipopolysaccharides(LPS)group(intravenous injection of LPS 5 mg/kg); low dose of propofol group(2 mg/kg induced and 4 mg·kg1·h-1 maintained); large dose of propofol group(4 mg/kg induced and 8 mg·kg-1 ·h-1 maintained).Six rats were killed at each time point after LPS intravenous administration(saline in group A)and the lungs were harved and the activation of Phospho c-Jun terninal kinase(p-JNK)was recorded in the lung tissues by the method of western-blot and Immunocytoche-mical(IHC).Results Western-blot and IHC shown that the activation of JNK in the lung tissues increased significantly after LPS administration(P=0.02-0.03＜0.05 or P=0.006-0.008＜0.01).The number ofp-JNK was 322±32 and 21.7±4.4 shown by western-blot and IHC at the time point of 3 h after LPS administration.The p-JNK at all time points declined significantly after treatment with propofol in group C and group D and the p-JNK declined to 235±26 and 179±21(western-blot)and 15.1±3.1 and 12.3±1.7(IHC)at the time point of 3 h.Compared with group C,p-JNK declined more than those in group D at the time point of 3 h after after LPS administration.Conclusion Treatment with propofol can significantly attenuate the p-JNK expression in LPS-induced acute lung injury in rats.