|
|||||
|
|
| 人MASP-2N端片段的原核表达 | |
| 引用本文： | 刘洁,李伟,姜文静,贾天军,常晓彤. 人MASP-2N端片段的原核表达[J]. 国际免疫学杂志, 2011, 34(6): 381-383,396. DOI: 10.3760/cma.j.issn.1673-4394.2011.06.014 |
| 作者姓名： | 刘洁 李伟 姜文静 贾天军 常晓彤 |
| 作者单位： | 1. 河北北方学院医学检验学院,张家口,075000 2. 河北北方学院附属一院骨外科,张家口,075000 |
| 基金项目： | 河北省科技厅自然基金课题，张家口市科学技术与发展计划项目 |
| 摘 要： | 目的 克隆并表达人MASP-2N端片段,为制备单克隆抗体及其临床应用奠定基础.方法 采用RT-PCR技术从人胎肝组织总RNA中扩增人MASP-2N端cDNA,克隆入pGEX-6p-2表达载体,在E.coli中表达GST- MASP-2N融合蛋白.结果 酶切图谱分析和DNA测序分析表明,成功构建了含人MASP-2N端片... |
| 关 键 词： | MASP-2N RT-PCR 基因克隆 原核表达 |
Prokaryotic expression of the N-terminal Fragment of human mannose- binding lectin associated Serine Protein Kinase-2 |
|
| LIU Jie,LI Wei,JIANG Wen-jing,JIA Tian-jun,CHANG Xiao-tong. Prokaryotic expression of the N-terminal Fragment of human mannose- binding lectin associated Serine Protein Kinase-2[J]. International Journal of Immunology, 2011, 34(6): 381-383,396. DOI: 10.3760/cma.j.issn.1673-4394.2011.06.014 | |
| Authors: | LIU Jie LI Wei JIANG Wen-jing JIA Tian-jun CHANG Xiao-tong |
| Affiliation: | .( College of Medicine Lab, HeBei North University, ZhangJiakou 075000, China) |
| Abstract: | Objective To clone and express the human MASP-2N-terminal fragment in E.coli.Methods Total RNA was extracted from human fetal liver tissue and MASP-2N cDNA was amplified by RT-PCR.The PCR product was cloned into pGEX-6p-2 vector.E.coli was transformed by the recombinant plasmid and GST- MASP-2N fusion protein was expressed.Results The constructed recombinant plasmids were identified by enzyme digestion and DNA sequencing.The recombinant protein was expressed in E.coli.Conclusion The N-terminal fragment of human MASP-2 was cloned and expressed. |
| Keywords: | MASP-2N RT-PCR G ene cloning Prokaryotic expression |
| 本文献已被 维普 万方数据 等数据库收录！ | |