Ghrelin对慢性充血性心力衰竭兔三层心肌间Cx43表达的影响

目的 观察生长激素释放肽(ghrelin)对慢性充血性心力衰竭(chronic congestive heart failure,CHF)兔左心室三层心肌间跨室壁缝隙连接蛋白43(connexin43,Cx43)表达的影响,为CHF恶性室性心律失常(malignant ventricular arrhythmia,MV...

Effect of Ghrelin on the expression of Cx43 in three layers in rabbit with chronic congestive heart failure

Objective To observe the effect and significance of Ghrelin on the expression of connexin43(Cx43) in three layers of left ventricular myocardium in rabbit with chronic congestive heart failure(CHF).Methods Forty New Zealand white rabbits were randomly divided into sham operation group(Sham group,n=20) and CHF group(n=20).In the CHF group,the CHF model was constructed by narrowing the abdominal aorta,and the sham group was not ligated.After 8 weeks of feeding,the left ventricular end-diastolic diameter(LVEDD),left ventricular posterior wall thickness(LVPW) and left ventricular ejection fraction(LVEF) were compared by in vivo echocardiography between the two groups.The heart rate(HR),peripheral arterial systolic pressure(SBP),diastolic blood pressure(DBP),mean arterial pressure(MAP),left ventricular maximum ascending velocity,and maximum descending velocity(±dp/dtmax) were assessed by haemodynamic measurements between the two groups.After 8 weeks of feeding,the Sham components were divided into Sham tyrode perfusion subgroup,Sham Ghrelin perfusion subgroup,CHF fraction into CHF tyrode perfusion subgroup,CHF Ghrelin perfusion subgroup,and rabbits were sacrificed to make left ventricular myocardial Masson staining images,respectively.Left ventricular endocardial(Endo),middle myocardium(Mid),and subepicardial myocardium(Epi) were excised from the heart and spleen of CHF group and Sham group,Expressiom levels of Cx43 protein(Cx43-pro)and mRNA(Cx43-Cq) in all three layers and the difference between the maximum Cx43-pro and the minimum Cx43-pro between the three layers of myocardium(ΔCx43-pro) and the maximum Cx43-Cq and minimum The difference between Cx43-Cq(ΔCx43-Cq) were measured by Western blotting and RT-PCR.Results Compared with Sham group,P,SBP,DBP and MAP in CHF group were significantly increased,LVEF were significantly decreased(P<0.05).Compared with the Sham tyrode perfusion subgroup,the expression of Cx43-pro and Cx43-Cq in the three layers myocardium of CHF tyrode perfusion subgroup was significantly lower(P<0.05).The expression of Cx43-pro and Cx43-Cq in CHF Ghrelin perfusion subgroup was significantly higher than that in CHF tyrode perfusion group,while △Cx43-pro and △Cx43-Cq were significantly lower than CHF desktop perfusion subgroup(P< 0.05).Conclusion The Cx43 protein in the left ventricular three-layer myocardium of CHF rabbits was reconstituted,and the content was significantly reduced.,and Ghrelin isolated pericardial fluid could improve the remodeling of Cx43-pro in rabbits with chronic congestive heart failure.