大鼠脑室触液神经元的分布及其在中缝核团与NADPH-d的共存

目的系统研究脑内接触脑脊液神经元的分布,并对远位触液神经元与一氧化氮合酶的共存进行研究。方法将霍乱毒素B(CTB)注入侧脑室,5d后将动物进行灌注,留取全脑切片,进行CTB免疫组化染色和NADPH鄄d组化染色,镜下观察免疫阳性细胞和纤维的分布。结果CTB标记的触液神经元的分布分为3个区:脑室壁周围、蛛网膜下及大脑皮质表层以及脑实质内。在中缝背核、中缝正中核和线形核等部位还可见NADPH鄄d阳性细胞以及CTB/NADPH鄄d双标细胞。结论侧脑室内触液神经元分布广泛;在中缝系统部分NADPH鄄d阳性细胞与远位触液神经元的共存,提示一氧化氮在脑鄄脑脊液之间的信息传递中有一定作用。

The distribution of cerebrospinal fluid contacting neurons (CSF-CN) and its coexistence with NADPH-d in raphe nuclei

Objective To investigate the distribution of cerebrospinal fluid contacting neurons (CSF-CN) in lateral ventricles and coexistence of distal CSF-CN with NADPH-d. Methods Cholera toxin subunit B was microinjected into lateral ventricles. Five days later, the rats were perfused. The brain slices were processed for CTB immunohistochemistry and NADPH-d histochemistry. Results The CTB-positive cells and fibers were mainly distributed in three areas: the walls of ventricles, surface of cerebrum and subarachnoid and the parenchyma of the brain. NADPH-d positive cells and NADPH-d/CTB double label cells can be detected in dorsal raphe nucleus(DR), median raphe nucleus (MnR) and caudal linear nucleus raphe (Cli). Conclusion The cerebrospinal fluid contacting neurons distributed very extensively. The coexistence of NADPH-d cells and CTB positive cells in raphe system suggested that nitric oxide (NO) may play a role in the transmission between cerebrum and cerebrospinal fluid.