急性白血病患者Bcl-2／Jh基因的研究

目的 探讨急性白血病（AL）患者t（14；18）融合基因Bcl-2／Jh突变点，在AL诊治过程中的变化及预后判断的意义。方法 采集AL患者和正常人外周血，PCR方法检测Bcl-2／Jh基因3种不同重排方式的发生率。结果 完全缓解期白血病患者体内mbr、mcr和mdr的发生率分别为46．6％、41．2％和13．8％。发生3种易位的机率为1．72％、2种易位的机率为5．2％。结论 Bcl-2／Jh在AL患者治疗前后的发生率和重排率虽有不同，可在一定程度上反映疗效。

Detection of Bcl - 2/IgH Fusion Gene of Acute Leukemia Patients

Objective To detect the Bcl-2/Jh fusion gene caused by t (14;18) translocation in acute leukemia patients and evaluate the clinical impli cations of bcl-2/Jh gene rearrangement in the diagnosis and therapy of leukemia. Methods Three types of Bcl-2/Jh gene rearrangement analysis in fresh peripheral blood samples was performed in patients with leukemia or heal thy people by polymerase chain reaction(PCR). Results The appea rance of bcl-2/Jh gene rearrangement rate was mbr 46.6%,mcr41.2%and 13.8% in pat ients with leukemia in remission The sumultaneity appearance of three types gene rearrangement was 1.72% respectively, the sumultaneity appearance of two was 5. 2%. Condusions Although the bcl-2/Jh gene rearrangement has dif ferent rate fore-and aft chemotherapy in acute leukemia,it may reflect the effec t to some extend ,further large sample quantitive analysis should be conducted t o be used in clinical diagnosis and monitoring prognosis.