精浆蛋白电泳分析的临床意义

目的为了初步了解男性精浆中的蛋白电泳谱以及蛋白谱与男性生育能力的相关关系。方法通过应用SPIFE3000全自动电泳分析仪对20例对照组(生育组)与140例观察组(不育组)的精浆进行蛋白分离(琼脂糖区带电泳法),建立其精浆蛋白质的特征电泳图谱,并进行对比分析。结果精浆蛋白经酸蓝染色后,区带清晰,从阳极到阴极分为A、B、C、D、E 5条带,电泳各区带百分比含量结果经方差分析,发现不育组与生育组A、C、D、E百分比含量变化有统计学意义(P<0.01),且无精子症患者精浆的蛋白谱有特异的"双峰"特点。结论精浆蛋白区带电泳分析可作为不育症的辅助诊断指标,且将是对男性不育诊断手段的有力补充。

Clinical application of protein electrophoresis in seminal plasma

Objective To understand the proteinogram of seminal plasma protein and the relationship between proteinogram and male fertility.Methods SPIFE3000 automatic electrophoretic analyzer and agarose gel electro- phoresis were applied to separate the seminal plasma protein.Twenty cases of control group(fertile group)and 140 cases of observation group(infertile group)were observed,and the characteristic proteinograms of seminal plasma protein were described and analyzed.Results After separated ,seminal plasma protein was dyed by acid-blue stain. Five clear stripes(A,B,C,D,E)were observed from anode to cathode.Analysis of variance was applied to analyse the percentage data of five stripes.Significant difference of the percentage data of four stripes(A,C,D,E) was found between fertile groups and infertile groups(P<0.01).Moreover,the characteristic of"two-humped"was observed in the proteinogram of seminal plasma protein in patients with azoospermatism.Conclusion The zonal electrophore- sis of seminal plasma protein can be one of markers of auxiliary diagnosis for male infertility.