| 大鼠磨牙实验性牙髓炎动物模型的建立 |
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| 引用本文： | 兰卫东,解保生,谭葆春,吴恩格. 大鼠磨牙实验性牙髓炎动物模型的建立[J]. 昆明医学院学报, 2004, 25(2): 10-14 |
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| 作者姓名： | 兰卫东 解保生 谭葆春 吴恩格 |
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| 作者单位： | 昆明医学院口腔学院,昆明,650031 |
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| 基金项目： | 云南省教育厅科研基金项目 ( 0 0 1 1 3 3 1 ) |
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| 摘 要： | 目的：探讨建立实验性牙髓炎动物模型的具体实验方法。方法：选用32只雄性Wistar大鼠，每只鼠均分为实验组及对照组,实验组牙髓暴露后用内毒素(LPS)处理，而对照组则用生理盐水(NS)处理,分别于术后1，3，5，7d和2，3，4，5周处死动物，获取下颌骨及牙齿标本，经固定、脱钙后作5μm厚的连续切片，HE染色后光镜观察。结果：LPS处理组牙髓组织发生典型的炎症变化。结论：LPS法诱导大鼠实验性牙髓炎简便可行。
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| 关 键 词： | 大鼠 牙髓炎 动物模型 建立 内毒素 |
| 文章编号： | 1003-4706(2004)02-0010-05 |
| 修稿时间： | 2004-01-10 |
Establishment of an Experimental Model of Rat Molars Pulpitis Induced by Lipopolysaccharide |
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| LAN Wei-dong,XIE Bao-sheng,TAN Bao-chun,WU En-ge. Establishment of an Experimental Model of Rat Molars Pulpitis Induced by Lipopolysaccharide[J]. Journal of Kunming Medical College, 2004, 25(2): 10-14 |
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| Authors: | LAN Wei-dong XIE Bao-sheng TAN Bao-chun WU En-ge |
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| Abstract: | Objective:The purpose of this study was to investigate the method of establishing an experimental model of rat molars pulpitis induced by lipopolysaccharide(LPS). Method:Thirty-two male,Wistar rats were used.The teeth in each rat were divided equally into experimental and control groups,and treated with LPS and 0.9%NS,respectively.The rats were sacrificed at 1,3,5,7 days,2,3,4,and 5 weeks.The mandible and teeth of each animal were fixed,decalcified,embedded in paraffin,and sectioned serially.The sections were stained with hematoxylin-eosin and examined under a light microscope.Result:The typical pulpitis was observed in experimental groups.Conclusion:The method to establish a rat pulpitis induced by LPS was feasible. |
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| Keywords: | Rat Pulpitis Lipopolysaccharide Animal model |
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