UF-500i、干化学和镜检法三种方法检测尿沉渣的对比研究及影响因素分析

目的：比较Sysmex UF-500i尿沉渣分析仪、干化学分析仪及尿沉渣镜检三种方法对尿中红细胞、白细胞检测的敏感性及其影响因素.方法：选取我院住院及门诊患者尿液974份,分别用UF-500i尿沉渣分析仪、干化学分析仪及尿沉渣镜检三种方法进行比较.结果：UF-500i检测出RBC、WBC的阳性率分别为31.2%、27.5% 干化学检测出RBC、WBC的阳性率分别为28.3%、16.2% 尿沉渣镜检检测出RBC、WBC的阳性率分别为35.9%、45.2%.干化学检出率较低.结论：UF-500i、干化学法和尿沉渣镜检三种方法联合应用,能有效排除假阳性和假阴性结果,减少检验误差,提高尿液分析质量.

Comparative study on UF--500i automated urinalysis analyzer, urine dipsticks analyzer and microscopy examination for detecting urinary sediments and analyze related influential factors

Objective：To compare the sensitivity and influencing factors in detecting urine erythrocyte and leukocyte by using Urine flow cytometer Sysmex UF--500i, urine dipsticks analyzer and sediment microscopy. Methods：974 cases urine examples were respectively examined by UF--500i, urine dipsticks analyzer and microscopy. Results：Analyzed by UF--500i,the positive rate of urine erythrocyte and leukocyte were 31.2 % and 27.5 %respectively;Analyzed by urine dipsticks analyzer, the positive rate of urine erythrocyte and leukocyte were 28.3 % and 16.2 % respectively;Analyzed by sediment microscopy,the positive percentage of urine erythrocyte and leukocyte were 35.9% and 45.2% respectively. The positive rate of urine dipsticks analyzer was lower compared with UF--500i and sediment microscopy. Conclusions： Combination of UF--500i automated urinalysis analy- zer, urine dipsticks analyzer and microscopy examination can avoid false positive and false negative results effectively,improve the accuracy and precision in urine analysis.