咳喘宁对支气管哮喘大鼠肺组织MMP-9mRNA表达的影响

目的观察咳喘宁对支气管哮喘大鼠肺组织MMP-9 mRNA表达的影响。方法取SD大鼠40只,随机分为正常对照组、模型对照组、咳喘宁高、低剂量组(27,13.5 g/kg)和桂龙咳喘宁组(0.41 g/kg),每组8只。除正常对照组外,其余各组以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各给药组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4周后处死大鼠,采用RT-PCR法检测MMP- 9 mRNA的表达,HE染色法观察肺和支气管病理组织学变化。结果与正常对照组相比较,模型对照组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织MMP-9 mRNA表达均明显增加(P<0.01);与模型对照组比较,各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及MMP-9 mRNA的表达(P<0.01)。结论咳喘宁能够降低肺组织MMP-9 mRNA的表达,抑制气道炎症和气道重塑。

Effect of Kechuan Ning on the Expression of MMP-9mRNA in Lung Tissue of Bronchial Asthmatic Rats

Objective To observe effect of Kechuan Ning (KN) on the expression of MMP-9mRNA in lung tissue of bronchial asthmatic rats.Methods Forty rats were randomly divided into 5 groups:the normal group,the model group,high-dose KN group (27 g/kg),low-dose KN group(13.5 g/kg),and Guilong Kechuan Ning group (0.45 g/kg),8 rats in each group.The bronchial asthmatic model was established by ovalbumin sensitization and long-term inhalation provocation.The rats in treatment groups received gastric gavage each day from the first time of provocation. After treatment for 4 weeks,the rats were killed and the pathological changes of lung tissue were observed after HE stain. The expression of MMP-9mRNA in lung tissue was determined by RT-PCR.Result Compared with the normal group,the thickness of bronchial wall and bronchial smooth muscle,the numbers of eosinophils and lymphocytes,and expression of MMP-9mRNA in lung tissue were increased(P<0.01);Compared with the model group,the thickness of bronchial wall and bronchial smooth muscle,the numbers of eosinophils and lymphocytes,and the expression of MMP-9mRNA in lung tissue were decreased in the treatment groups(P<0.01).Conclusion Kechuan Ning could de- crease the expression to MMP-9mRNA to restrain airway remodeling and inflammation.