Favourable associations between the myosin heavy-chain and light-chain isoforms in human skeletal muscle |
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| Authors: | Masanobu Wada Shigeru Katsuta Tokuhide Doi Shin -ya Kuno |
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| Affiliation: | (1) Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89 Naka-ku, Hiroshima-shi, Hiroshima-ken, Japan;(2) Institute of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki-ken, Japan;(3) Tokyo Metropolitan Prosthetic and Orthotic Research Institute, 3-17-3 Toyama, Shinjuku-ku, Tokyo-to, Japan;(4) Department of Radiology, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki-ken, Japan |
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| Abstract: | Histochemical and biochemical analyses were performed in order to examine the relationship between myosin light-chain (LC) isoforms and fibre-type distributions in whole human skeletal muscle. Muscle biopsies were obtained from the vastus lateralis muscle in six healthy men, and analysed for the relative area occupied by each fibre type (percentage of fibre type area) and the molar ratio of each LC isoform. The percentage of type I fibre area was positively correlated with the molar ratio of slow LC (LC1s and LC2s) to total LC. The regression line was located below the line of unity. Also, the ratio of percentage of type II fibre area to that of type II fibre area was positively correlated with the molar ratio of the fast alkali LC LC1f to fast alkali LCs LC1f and LC3f. These results support previous study, having shown that in human skeletal muscle some type I fibres express various amounts of fast LC in addition to slow LC and suggest that fast myosin heavy-chain HCII a is favourably associated with LC1f, whereas HCIIb is favourably associated with LC3f. |
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| Keywords: | Skeletal muscle Myosin light chain Myosin heavy chain Fibre type Isoforms Percentage fibre type area Favourable association |
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