高血压病患者阿司匹林抵抗与β纤维蛋白原基因启动子区-455G／A多态性的关系

目的探讨高血压患者β纤维蛋白原基因启动子区-455G／A多态性与阿司匹林抵抗的关系。方法用电阻抗法测定108例高血压患者口服阿司匹林前后ADP及花生四烯酸诱导的血小板聚集电阻抗值（ohm），判定患者阿司匹林疗效，分为阿司匹林敏感、环氧化酶型阿司匹林抵抗，环氧化酶旁路型阿司匹林抵抗；用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法检测βFg基因启动子区-455G／A多态性，依据多态性结果分为GG基因组和GA＋AA基因组，比较不同基因组纤维蛋白原浓度以及阿司匹林抵抗发生率。结果高血压患者GG、GA、AA基因型频率分别为0．639、0．324、0．037，G、A等位基因频率分别为0．801、0．199。GA＋AA组纤维蛋白原浓度高于GG组（3．30±0．98vs2．93±0．70，p〈0．05）。环氧化酶型阿司匹林抵抗发生率GG组、GA＋AA组分别为13．04％、7．69％（Х^2=0．282，p〉0．5）；环氧化酶旁路型阿司匹林抵抗发生率GA＋AA组显著高于GG基因组（61．54％vs34．78％，Х^2=5．841，p=0．016）。结论βFg基因-455G／A启动子区携带A-455等位基因高血压患者环氧化酶旁路型阿司匹林抵抗发生率高，A-455等位基因与环氧化酶旁路型阿司匹林抵抗具有相关性。

The Relationship of Beta-fibrinogen Gene -455G/A Polymorphism and Aspirin Resistance in Hypertension Patients

Objective To investigate the relationship between -455G/A polymorphisms on the promoter region of β Fg gene and aspirin resistance in hypertension patients. Methods The platelet aggregation function before and after taking aspirin was measured by whole blood imped- ance method in 108 patients. The -455G/A polymorphismof β Fg was screened by PCR-RFLP. The criteria of aspirin resistance has been described previously. The types of aspirin resistance include COX-AR and COX-AP-AR. According to the -455G/A polymorphism of β Fg gene, AA patients were few. So all patients were divided into two gene groups： GG group and GA＋AA group, then we compared fibfinogen concentration and aspirin resistance incidence between two groups. Results The frequencies of A^-455, G^-455allele were 0.199 and 0.801 respectively and the frequencies of the genotype GG, GA, AA were 0.639, 0.324 and 0.037 respectively for hypertension patients. Fibrinogen in group GA＋AA was higher than in group GG （3.30±0.98vs2.93 ±0.70,p〈0.05） ; COX-AP-AR incidence of GA ＋ AA group was higher than GG group （61.54% vs 34.78%, Х^2=5.841, p=0.016）. Conclusions the A^-455 allele on the promoter pegion of β -fibrinogen gene in hypertension patients is associated with COX-AP-AR.