食管癌RUNX3蛋白的表达特点及意义

[目的]了解RUNX3蛋白在食管癌中的表达及病理学意义.[方法]将手术切除癌旁相对正常食管组织9例、癌前病变38例与食管癌组织83例制成石蜡包埋组织微阵列,用免疫组织化学方法检测RUNX3的表达,并分析其表达与食管癌侵袭及转移的关系.[结果]癌旁、癌前病变到食管癌组织RUNX3蛋白表达率分别为77.8％、78.9％与42.2％.食管癌组显著低于癌旁相对正常组、癌前病变组(P＜0.05).食管癌中RUNX3蛋白在淋巴结转移组表达率为22.7％,显著低于无淋巴结转移组的64.1％ (P＜0.05),浸润深度T3、4组(31.0％)显著低于T1、2组68.0％ (P ＜0.05);Ⅲ-Ⅳ期患者组(32.5％)低于Ⅰ-Ⅱ期组(51.2％),但差异无显著性意义(P＞0.05).[结论] RUNX3蛋白表达下调可能与食管癌的发生发展有关.

Down regulation of RUNX3 expression in esophageal cacinoma and its significance

[Objectives] To profile the expression characteristics of RUNX3 in esophageal carcinogenesis and explore its pathological significance.[Methods] Nine relative normal esophagus,38 precancerous lesion and 83 esophageal carcinoma tissues(EC) without preoperative chemo/radiotherapy were selected to manufacture the paraffin embedded tissue array.The expression of RUNX3 was detected by immunohistochemistry,the correlation of RUNX3 expressions to EC invasiveness and metastasis were analyzed.[Results] The rates of RUNX3 expression in normal tissue,precancerous and EC were 77.8%,78.9% and 42.2%,respectively(P<0.05).There were significant differences between the EC group and noncancerous or precancerous lesion,the rate of EC group was significantly lower than those of normal and precancerous groups(P>0.05).The rate of lymphatic metastasis group 22.7% was significantly lower than that of without 64.1%(P<0.05) as well as the rate in stage T3,4 group(31.0%) was significantly lower than that in stage T1,2 group(68.0%)(P<0.05).The rate in clinical stage III-IV(32.5%) was lower than that in the clinical stage I-II(51.2%),but no significant difference was found(P>0.05).[Conclusion] The characteristics of RUNX3 expression is one of important molecular events throughout esophageal carcinogenesis and dissemination which is closely related to the development of EC.It may be a potencial biomarker in oncological molecular diagnosis and prognosis prediction for EC.