氨溴索对流感嗜血杆菌生物膜清除和膜内杀菌作用研究

目的：观察氨溴索（AMB）对流感嗜血杆菌生物膜（BF）的作用以及对BF菌的杀菌作用。方法：30株流感嗜血杆菌菌株从腺样体肥大患儿术中切除的腺样体组织分离，选取具有较强成膜能力的2株细菌，96微孔板法培养细菌形成BF，结晶紫染色法测定不同浓度AMB对2株细菌BF的影响，并用扫描电镜观察细菌BF形态结构的改变；平板计数法检测AMB对BF菌杀菌作用。结果：结晶紫染色法示，当AMB浓度分别达到0．25mg／ml和0．49mg／ml时，2株细菌BF吸光度值较对照组差异有统计学意义（P〈0．01），扫描电镜发现BF结构受到破坏；菌落计数显示AMB对流感嗜血杆菌BF菌有杀菌作用，且剂量依赖效果明显。结论：AMB在体外对流感嗜血杆菌BF具有破坏和清除功能，并对BF菌具有杀菌作用。

Effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action

Objective：To establish a biofilm model of Haemophilus influenzae and observe the effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action. Method： Thirty strains of Haemophilus influenzae were isolated from adenoids of children with adenoidal hypertrophy. Two strains which could build stronger biofilms was selected in a 96-well plate. The effect of ambroxol on biofilms were determined by crystal violet, and the structure of biofilms were observed by scanning electron microscope （SEM）. The numbers of viable bacterial in biofilm after ambroxol treatmented determined by plate culture count. Result： Through crystal violet assay, significant difference （P〈0.01） between the two group after treatment was found when ambroxol concentration reached at 0.25 mg/ml and 0.49 mg/ml. The biofilms was destroyed by SEM. Ambroxol had the positive effect on bacterial killing by plate culture count,and the effect was in a dose dependent. Conclusion： Ambroxol could destroy the biofilm of Haemophilus influenzae, and had bactericidal function in vitro.