基质金属蛋白酶-9基因多态性与喉癌易感性及预后的研究

目的：探讨MMP-9基因多态性与喉癌易感性及临床病理特征和预后的关系。方法：应用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法，检测142例喉癌患者和200例健康对照者MMP-9—1562C／T单核苷酸多态性位点的基因型。结果：携带MMP-9—1562CC基因型者患喉癌的风险高于携带MMP-9—1562cT／TT基因型者（P〈O．05，95％CI=1．02～2．77）；MMP-9-1562C／T基因多态性与喉癌患者年龄、性别、T分期、临床分期及淋巴结转移无相关性；Kaplan—Meier生存分析结果显示，MMP-9—1562CC基因型患者的生存率低于TT和CT基因型患者（P〈0．01）；COX回归分析显示，携带MMP-9—1562CC基因型的患者预后不良。结论：MMP-9—1562C／T基因多态性与喉癌的发病风险相关；MMP-9—1562c／T基因多态性可能是影响预后的独立危险因素。

Correlation of MMP-9 polymorphism with susceptibility and prognosis of laryngeal carcinoma

Objective ： To investigate the correlation of MMP-9 single nucleotide polymorphism（SNP） with susceptibility ,clinicopathological characteristics and prognosis of laryngeal carcinoma. Method.. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to detect the MMP-9-1562C/T SNP genotype in 142 patients with laryngeal carcinoma and 200 healthy controls. Result： The MMP-9-1562CC genotype significantly increased the risk for laryngeal carcinoma compared to MMP-9-1562CT/TT genotype（P〈0.05,95% CI=1. 02-2.77）. MMP-9-1562C/T polymorphism did not correlate to age, sex, T stage, N stage and clinical stage. Univariate analysis indicated that the overall survival rate in patients with MMP-9-1562CC genotype was lower than that in MMP-9-1562CT/TT genotype. Multivariate analysis indicated that the patients with MMP-9- 1562CC genotype had unfavourable prognosis. Conclusion.. MMP-9-1562C/T polymorphism was closely correlateed with the risk for laryngeal carcinoma, and it may be an independent prognostic factor.