| Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoa protein in human normal motility sperm and idiopathic asthenospermia |
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| 引用本文： | 申树林,贺大林,罗勇,宁亮. Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoa protein in human normal motility sperm and idiopathic asthenospermia[J]. 中国人民解放军军医大学学报, 2006, 21(5) |
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| 作者姓名： | 申树林 贺大林 罗勇 宁亮 |
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| 作者单位： | Department of Urology First Affiliated Hospital，Medical School，Xi'an Jiaotong University，Department of Urology，First Affiliated Hospital，Medical School，Xi'an Jiaotong University，Department of Urology，First Affiliated Hospital，Medical School，Xi'an Jiaotong University，Department of Urology，First Affiliated Hospital，Medical School，Xi'an Jiaotong University，Xi'an 710061，China，Xi'an 710061，China，Xi'an 710061，China，Xi'an 710061，China |
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| 摘 要： | Objective: To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in the human asthenospermia. Methods: Two-dimensional gel electrophoresis was performed on 4 normal sperm samples from healthy men and 4 sperm samples from 4 asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software. Results: Six differential protein spots were identified. Four spots showed increased expression in the control gels compared with the patient gels. Conclusion: The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data of this study would establish the better fundament for further isolation and identification of differentially expressed proteins in human asthenospermia sperm.
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Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoa protein in human normal motility sperm and idiopathic asthenospermia |
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| SHEN Shu-lin,HE Da-lin,LUO Yong,NING Liang. Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoa protein in human normal motility sperm and idiopathic asthenospermia[J]. Journal of Medical Colleges of PLA(China), 2006, 21(5) |
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| Authors: | SHEN Shu-lin HE Da-lin LUO Yong NING Liang |
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| Affiliation: | Department of Urology,First Affiliated Hospital,Medical School,Xi'an Jiaotong University,Xi'an,710061,China Department of Urology,First Affiliated Hospital,Medical School,Xi'an Jiaotong University,Xi'an,710061,China Department of Urology,First Affiliated Hospital,Medical School,Xi'an Jiaotong University,Xi'an,710061,China Department of Urology,First Affiliated Hospital,Medical School,Xi'an Jiaotong University,Xi'an,710061,China |
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| Abstract: | Objective: To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in the human asthenospermia. Methods: Two-dimensional gel electrophoresis was performed on 4 normal sperm samples from healthy men and 4 sperm samples from 4 asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software. Results: Six differential protein spots were identified. Four spots showed increased expression in the control gels compared with the patient gels. Conclusion: The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data of this study would establish the better fundament for further isolation and identification of differentially expressed proteins in human asthenospermia sperm. |
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| Keywords: | idiopathic asthenospermia protein two-dimensional gel electrophoresis |
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