| 重型脑损伤大鼠脑组织蛋白质组差异表达 |
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| 引用本文： | 张赛,刘振林,孙洪涛,胡群亮,孙世中,刘晓智.重型脑损伤大鼠脑组织蛋白质组差异表达[J].中华创伤杂志,2008,24(6). |
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| 作者姓名： | 张赛 刘振林 孙洪涛 胡群亮 孙世中 刘晓智 |
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| 作者单位： | 武警医学院附属医院神经外科,天津市蛋白组学重点实验室,天津,300162 |
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| 摘 要： | 目的 寻找重型脑损伤(severe traumatic brain injury,sTBI)大鼠伤后不同时间的蛋白质组变化规律.方法 提取创伤后3,7和14 d的鼠脑组织总蛋白进行双向凝胶电泳,筛选和鉴定差异蛋白质点.结果 共筛选出17个差异蛋白质点,它们参与细胞代谢、应激与炎症反应等过程.结论 利用双向凝胶电泳技术可寻找到多种参与重型脑损伤病程演进的蛋白质.
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| 关 键 词： | 脑损伤 双向凝胶电泳技术 蛋白质组 |
Differential expression of poroteome in brain tissues from rats with severe traumatic brain injury |
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| ZHANG Sai,LIU Zhen-lin,SUN Hong-tao,HU Qun-liang,SUN Shi-zhong,LIU Xiao-zhi.Differential expression of poroteome in brain tissues from rats with severe traumatic brain injury[J].Chinese Journal of Traumatology,2008,24(6). |
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| Authors: | ZHANG Sai LIU Zhen-lin SUN Hong-tao HU Qun-liang SUN Shi-zhong LIU Xiao-zhi |
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| Abstract: | Objective To study the changes of proteome expression in brain tissues from rats with severe traumatic brain injury(sTBI). Methods Total protein of brain tissues were obtained at days 3,7 and 14 for two-dimensional gel electrophoresis to screen and identify differential protein spots.Results We screened 17 differential protein spots that were involved in cellular metabolism,stress and inflammatory reaction. Conclusion Some differential proteins involved in sTBI can be found by twodimensional gel electrophoresis. |
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| Keywords: | Brain injuries Two-dimensional gel dectrophoresis Proteome |
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