十二指肠NaCl刺激对大鼠孤束核c-fos表达的影响

目的：探讨生理状态下，大鼠十二指肠受到不同浓度氯化钠刺激后，孤束核（NTS）中神经元的活动性。方法：在大鼠十二指肠内恒流灌注0.86mol／L NaCl、0.15mol/L NaCl、去离子水以及10^-4mol／L 5-HT，然后对各处理组中不同的NTS部位进行c-fos免疫组化，并作定量分析。结果：0.86mol／L NaCl以及5-HT组可以引起NTS各平面c-fos阳性神经元数量的增加，而且明显高于0.15mol／L NaCl组、去离子水组以及假手术组。结论：在大鼠十二指肠给予0.86mol／L NaCl，可以经迷走神经通路上传到NTS，由NTS整合内脏信息上传至更高一级中枢；而给予0.15mol／L NaCl和去离子水，则不能激活NTS内的内脏感觉神经元。

Effect of duodenal stimulation with sodium chloride on c-fos expression in nucleus of solitary tract in rats

Objective: To investigate the activities of visceral sensory neurons in nucleus of solitary tract after receiving the duodenal stimulation (sodium chloride) in rats. Methods: Rats were divided into 5 groups: NaCl (0. 86 mol/L), NaCl (0. 15 mol/L), deionized water, 5-HT (10-4 mol/L) and sham operation group. Immunohistochemical staining and quantitative analysis of c-fos were done in all kinds of groups and regions (N1, N2, N3, N4, N5). Results: NaCl (0. 86 mol/L) and 5-HT (10-4 mol/L) groups had strong c-fos-like immunoreactivity in NTS (P<0. 01) compared with all the other groups. Conclusion: NaCl (0. 86 mol/L), as the duodenal stimulation, activates visceral sensory neurons in NTS, which processes those information and transmit them. NaCl (0. 15 mol/L) and deionized water do not activate the neurons in NTS.