丹皮酚协同顺铂对人卵巢癌细胞A2780s的增殖抑制及促凋亡作用

目的:探讨丹皮酚(Pae)单药及联合顺铂(CDDP)对人卵巢癌细胞A2780s的增殖抑制,凋亡诱导作用及对细胞周期分布的影响.方法:以不同浓度的Pae、CDDP和两药联合作用于人卵巢癌细胞A2780s,采用MTT法测定药物对体外培养的人卵巢癌细胞A2780s增殖的抑制作用,应用两药相互作用指数(CDI)进行联合用药分析...

Effect of Paeonol plus Cisplatin on Anti-Proliferation and Apoptosis of Human Ovarian Carcinoma A2780s Cells

Objective: To explore the effect of paeonol comblined with cisplatin on proliferation inhibition, apoptosis and cell cycle distribution of human ovarian carcinoma A2780s Cells. Methods: The inhibitory effect of paeonol or cisplatin alone or both with different concentrations on A2780s in vitro was measured by MTT assay. The coefficient of drug interaction (CDI) was used to analyze the synergistical inhibitory effect of drug combination. The apoptosis and cell cycle distrubition was analyzed by flow cytometry. Results: Paeonol or cisplatin alone had anti-proliferative effect on A2780s cells in a dose-and time-dependent manner. The synergistic effect (CDI<0.7) was significant after treatment with a combination of paeonol (62.5 mg/L) with CDDP (5 mg/L). The apoptosis rate of the combined group (44.9%) was higher than the groups treated with paeonol or cisplatin respectively. Conclusion: Paeonol and cisplatin can inhibit the proliferation of human ovarian cancer cell A2780s and induce apoptosis, and the combination of paeonol and cisplatin have synergistic anti-tumor effect.